A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
复制标题
一种用于研究大鼠固定诱发膝关节屈曲挛缩的微创内固定技术
DOI：
10.3791/59260
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发表时间：
2019
影响因子：
1.2
通讯作者：
Wang Kun
中科院分区：
文献类型：
--
作者：
Jiang Shihai;Yi Xiaoyou;Luo Yuansen;Yu Dongjie;Liu Yuangao;Zhang Fei;Zhu Lei;Wang Kun
关节挛缩是骨科常见的并发症之一，是由于长期的关节制动所造成。目前，利用内固定来限制膝关节活动度是一种被广泛接受的产生实验性挛缩的模型。然而，植入应用不可避免地会对动物造成手术创伤。为了开发一种微创方法，我们在手术过程中将肌肉间隙分离方法与先前报道的小切口技术相结合：在大腿和小腿外侧做两个小皮肤切口，然后进行肌肉间隙分离以暴露骨表面。在不干扰重要神经或血管的情况下，通过预先构建的内固定在大约135°膝关节屈曲处逐渐固定大鼠膝关节。正如预期的那样，这种简单的技术允许动物快速术后康复。通过X线或显微CT扫描分析确认内固定的正确位置。与对侧膝关节相比，固定膝关节的活动范围明显受限，证明了该模型的有效性。此外，组织学分析显示，随着时间的推移，纤维沉积和粘连在后上膝关节囊的发展。因此，这种微创模型可能适合于模拟固定膝关节挛缩的发展。
Joint contracture, resulting from a prolonged joint immobilization, is a common complication in orthopedics. Currently, utilizing an internal fixation to restrict knee joint mobility is a widely accepted model to generate experimental contracture. However, implanting application will inevitably cause surgical trauma to the animals. Aiming to develop a less invasive approach, we combined a muscle-gap separation modus with a previously reported mini-incision skill during the surgical procedure: Two mini skin incisions were made on the lateral thigh and leg, followed by performing muscle-gap separation to expose the bone surface. The rat knee joint was gradually immobilized by a preconstructed internal fixation at approximately 135° knee flexion without interfering essential nerves or blood vessels. As expected, this simple technique permits rapid postoperative rehabilitation in animals. The correct position of the internal fixation was confirmed by an x-ray or micro-CT scanning analysis. The range of motion was significantly restricted in the immobilized knee joint than that observed in the contralateral knee joint demonstrating the effectiveness of this model. Besides, histological analysis revealed the development of fibrous deposition and adhesion in the posterior-superior knee joint capsule over time. Thus, this mini-invasive model may be suitable for mimicking the development of immobilized knee joint contracture.