Embryonal carcinoma cell induction of miRNA and mRNA changes in co-cultured prostate stromal fibromuscular cells.
Embryonal carcinoma cell induction of miRNA and mRNA changes in co-cultured prostate stromal fibromuscular cells.
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胚胎癌细胞诱导共培养的前列腺基质纤维肌肉细胞中 miRNA 和 mRNA 的变化。
DOI：
10.1002/jcp.22464
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发表时间：
2011
影响因子：
5.6
通讯作者：
Liu,AlvinY
中科院分区：
文献类型：
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作者：
Vêncio,EneidaF;Pascal,LauraE;Page,LauraS;Denyer,Gareth;Wang,AmyJ;Ruohola-Baker,Hannele;Zhang,Shile;Wang,Kai;Galas,DavidJ;Liu,AlvinY
前列腺间质间充质控制器官特异性发育。在癌症中，与非癌症相比，基质区室显示出改变的基因表达。癌症相关基质细胞和正常组织基质细胞之间的谱系关系尚不清楚。也不是表达差异的根本原因。以前，我们使用胚胎癌（EC）细胞系NCCIT来研究基质诱导特性。在本研究中，从组织标本中分离非癌症（NP）和癌症（CP）的基质细胞，并以trans-well格式与NCCIT细胞共培养，以排除异型细胞接触。3天后，通过基因阵列分析基质细胞的microRNA（miRNA）和mRNA表达。在共培养中，发现NCCIT细胞将NP基质细胞的miRNA和mRNA表达改变为与CP基质细胞相似的表达。NCCIT对CP基质细胞基因表达无明显影响。我们得出结论，基质细胞中的基因表达变化可以由EC细胞合成的扩散因子诱导，并表明癌症相关基质细胞代表更原始或分化程度更低的基质细胞类型。J.细胞。226：1479-1488，2011。© 2010 Wiley利斯公司
The prostate stromal mesenchyme controls organ‐specific development. In cancer, the stromal compartment shows altered gene expression compared to non‐cancer. The lineage relationship between cancer‐associated stromal cells and normal tissue stromal cells is not known. Nor is the cause underlying the expression difference. Previously, the embryonal carcinoma (EC) cell line, NCCIT, was used by us to study the stromal induction property. In the current study, stromal cells from non‐cancer (NP) and cancer (CP) were isolated from tissue specimens and co‐cultured with NCCIT cells in a trans‐well format to preclude heterotypic cell contact. After 3 days, the stromal cells were analyzed by gene arrays for microRNA (miRNA) and mRNA expression. In co‐culture, NCCIT cells were found to alter the miRNA and mRNA expression of NP stromal cells to one like that of CP stromal cells. In contrast, NCCIT had no significant effect on the gene expression of CP stromal cells. We conclude that the gene expression changes in stromal cells can be induced by diffusible factors synthesized by EC cells, and suggest that cancer‐associated stromal cells represent a more primitive or less differentiated stromal cell type. J. Cell. Physiol. 226: 1479–1488, 2011. © 2010 Wiley‐Liss, Inc.