Antibacterial and allelopathic activity of extract from Castanea sativa leaves
Antibacterial and allelopathic activity of extract from Castanea sativa leaves
复制标题
DOI：
10.1016/s0367-326x(00)00185-4
复制
发表时间：
2000-08-01
期刊：
影响因子：
3.4
通讯作者：
Ferrara, L
中科院分区：
文献类型：
--
作者：
Basile, A;Sorbo, S;Ferrara, L
用硫酸水溶液（pH 3.0）提取板栗（Castanea sativa）的乙酸乙酯可溶性组分，测定其抑菌和化感作用。该提取物显示出对所用的八种革兰氏阳性和革兰氏阴性细菌菌株中的七种具有显著的抗菌效果（MIC在64-256 μ g/ml的范围内，MBC在256-512 μ g/ml的范围内）。通过TLC和HPLC分析活性部分，显示存在芸香苷、橙皮苷、槲皮素、芹菜素、桑色素、柚苷、高良姜苷和山奈酚。针对相同的细菌菌株测试鉴定的黄酮类化合物的标准品。槲皮素、芦丁和芹菜素的活性最高。测定了该物质对萝卜种子萌发的化感作用。提取物、槲皮素、芦丁和芹菜素降低了种子的发芽率，降低了根和上胚轴的生长。(C)2000 Elsevier Science B. V.保留所有权利。
Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 mu g/ml and MBC in the range of 256-512 mu g/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth. (C) 2000 Elsevier Science B.V. All rights reserved.