Banded karyotype of Nelore cattle (Bos taurus indicus Linnaeus, 1758)
Banded karyotype of Nelore cattle (Bos taurus indicus Linnaeus, 1758)
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DOI：
10.3897/compcytogen.v13i3.36449
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发表时间：
2019-08-29
影响因子：
1
通讯作者：
da Cruz, Aparecido Divino
中科院分区：
文献类型：
--
作者：
Amancio, Andreia Pires;Moreira Duarte, Sabrina Sara;da Cruz, Aparecido Divino
染色体处理技术的应用和标准化，以获得核型特征的第一次在巴西的Nelore牛- Bos taurus indicus Linnaeus，1758年-（牛亚种最突出的巴西牲畜）。从戈亚斯天主教大学农业和生物科学学院的动物中采集血样，其中两只雄性和两只雌性为纯种动物。将这些样本提交至细胞培养方法，以研究中期染色体。染色体分带技术（C、G和NOR）揭示了尼罗牛的核型结构与以往核型分析的其他品种的瘤牛相同。二倍体染色体数目总是正常的，2n = 60。C显带显示几乎所有染色体的着丝粒区域都有C阳性异染色质。G-显带在相应的染色体对中呈现出预期的带型，与该物种已确定的染色体模式相一致。在7条常染色体长臂的端粒末端发现了核仁组成区（AgNOR）的银染。在这项研究中，我们发现更多的区域在染色体染色比文献中提出的牛indicus组（BIN）。这些NOR区域在所研究的4只动物的相同染色体上重复。
Chromosome handing techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle - Bos taurus indicus Linnaeus, 1758 - (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goias, two males and two females of pure breed. These samples were submitted to the cell culture method to study metaphase chromosomes. Chromosome banding techniques (C, G and NOR) revealed the karyotype architecture of Nelore cattle common with that of other breeds of zebu cattle formerly karyotyped. The diploid chromosome number was invariably normal, 2n = 60. C-banding revealed C-positive heterochromatin in centromeric regions almost in all chromosomes. G-banding presented the expected band pattern in the respective chromosome pairs in correspondence with the established chromosomal patterns for the species. Ag-staining for nucleolus organizer regions (AgNOR) was identified on the telomeric end of the long arm in 7 autosomal chromosomes. In this study we found more regions in chromosomes with staining than presented in the literature for the Bos indicus group (BIN). These NOR regions were repeated on the same chromosomes for the 4 animals studied.