Amplification and deregulation of MYC following Epstein-Barr virus infection of a human B-cell line.
Amplification and deregulation of MYC following Epstein-Barr virus infection of a human B-cell line.
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DOI：
10.1073/pnas.84.16.5838
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发表时间：
1987-08
影响因子：
11.1
通讯作者：
J. Lacy;W. P. Summers;M. Watson;P. Glazer;W. Summers
中科院分区：
文献类型：
--
作者：
J. Lacy;W. P. Summers;M. Watson;P. Glazer;W. Summers
在爱泼斯坦-巴尔病毒（EB V）阳性的伯基特淋巴瘤（BL）中，EB病毒在MYC癌基因易位和失调中的作用仍然未知。通过利用EBV阴性BL（BJAB）和通过体外感染从其衍生的几个EBV阳性亚系，有可能表明病毒的存在与MYC的表达和拷贝数的改变相关。在EBV阴性BJAB系中，随着细胞接近生长的稳定期，MYC转录物的水平逐渐下降。相反，在EBV感染的BJAB细胞中，MYC表达随着细胞进入稳定期而保持升高。这种对MYC表达的影响与病毒的存在可逆地相关。此外，两种不同的EBV株感染BJAB细胞后，MYC扩增与MYC定位位置处8号染色体上出现均匀染色区域相关。这些研究表明，MYC转录的失调和MYC基因座区域中的染色体重排在该B细胞系中可能由于EBV感染而发生。
In Epstein-Barr virus (EBV)-positive Burkitt lymphoma (BL) the role of EBV in the translocation and deregulation of the MYC oncogene remains unknown. By utilizing an EBV-negative BL (BJAB) and several EBV-positive sublines derived from it by in vitro infection, it was possible to show that the presence of the virus was associated with altered expression and copy number of MYC. In the EBV-negative BJAB line, the level of MYC transcripts declined progressively as cells approached the stationary phase of growth. In contrast, in EBV-infected BJAB cells MYC expression remained elevated as cells entered stationary phase. This effect on MYC expression was reversibly linked to the presence of the virus. Furthermore, following EBV infection of BJAB cells by two different strains of EBV, amplification of MYC in association with the appearance of a homogeneously staining region on chromosome 8 at the mapped location of MYC had occurred. These studies suggest that both the deregulation of MYC transcription and the chromosomal rearrangement in the region of the MYC locus in this B-cell line may have occurred as a result of EBV infection.