Determination of isolated myocyte viability: staining methods and functional criteria.
Determination of isolated myocyte viability: staining methods and functional criteria.
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分离的心肌细胞活力的测定：染色方法和功能标准。
DOI：
10.1007/978-3-662-11041-6_4
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发表时间：
1985
影响因子：
9.5
通讯作者：
Bonventre,JV
中科院分区：
文献类型：
--
作者：
Cheung,JY;Leaf,A;Bonventre,JV
从成年大鼠心脏成功分离出耐受生理水平钙的单个心室细胞。这些细胞表现出完整心肌的形态和功能特征。无论是在细胞外钙的情况下，或在存在6%PEG的厌氧孵育下，肌细胞排除台盼蓝，尽管在生化功能严重紊乱。我们认为台盼蓝进入可能是一个相当不敏感的细胞损伤的标准。我们建议将杆状形态、ATP水平和体外起搏下收缩的能力相结合，作为细胞活力的替代简单测试。
Single ventricular cells tolerant to physiological levels of calcium have been successfully isolated from adult rat heart. These cells exhibit morphological as well as functional characteristics of the intact myocardium. Under anaerobic incubations either in the absence of extracellular calcium or in the presence of 6% PEG, myocytes excluded Trypan blue despite severe derangements in biochemical functions. We suggest that Trypan blue entry may be a rather insensitive criterion of cell injury. We recommend the combination of rod-shape morphology, ATP levels and the ability to contract under external pacing as alternative simple tests of cell viability.