Basal foot MTOC organizes pillar MTs required for coordination of beating cilia.
Basal foot MTOC organizes pillar MTs required for coordination of beating cilia.
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基底脚MTOC组织了击败纤毛的协调所需的支柱。
DOI：
10.1038/ncomms5888
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发表时间：
2014-09-12
影响因子：
16.6
通讯作者：
Delacour D
中科院分区：
文献类型：
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作者：
Clare DK;Magescas J;Piolot T;Dumoux M;Vesque C;Pichard E;Dang T;Duvauchelle B;Poirier F;Delacour D
纤毛搏动的协调对确保气道粘液的清除至关重要。纤毛运动的定向和同步部分地响应底层细胞骨架网络的组织。通过对小鼠气管的电子断层扫描，我们发现基底体被由4-5个微管组成的规则微管阵列共同钩在皮层上。半乳糖凝聚素-3（一种基底体成分）的去除会引起γ-微管蛋白的错误募集，这种源自基底足帽的微管框架紊乱，同时导致基底体对齐和纤毛定向丧失，纤毛组织缺陷和气管腔内流体流动减少。我们得出结论，半乳糖凝集素-3在纤毛微管组织中心和“支柱”微管的维持中起着至关重要的作用，并且该网络对于运动纤毛的协调定向和稳定起着重要作用。
Coordination of ciliary beating is essential to ensure mucus clearance in the airway tract. The orientation and synchronization of ciliary motion responds in part to the organization of the underlying cytoskeletal networks. Using electron tomography on mouse trachea, we show that basal bodies are collectively hooked at the cortex by a regular microtubule array composed of 4-5 microtubules. Removal of Galectin-3, one of basal body components, provokes misrecruitment of γ-tubulin, disorganization of this microtubule framework emanating from the basal foot cap, together with loss of basal body alignment and cilium orientation, defects in cilium organization and reduced fluid flow in the tracheal lumen. We conclude that Galectin-3 plays a crucial role in the maintenance of the microtubule organizing center of the cilium and the “pillar” microtubules, and that this network is instrumental for the coordinated orientation and stabilization of motile cilia.