DUGBE VIRUS - TICK-BORNE ARBOVIRUS FROM NIGERIA
DUGBE VIRUS - TICK-BORNE ARBOVIRUS FROM NIGERIA
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DOI：
10.1099/0022-1317-23-3-297
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发表时间：
1974-01-01
影响因子：
3.8
通讯作者：
PORTERFI.JS
中科院分区：
文献类型：
--
作者：
DAVIDWES.TS;PORTERFI.JS
杜格贝病毒在猪和猴源细胞系中繁殖，产生的细胞病变效应很小，但在光学显微镜检查的染色制剂中，明显可见明显的核旁、细胞质包涵体。电镜下可见大量直径90 ~ 100 nm的病毒颗粒。在细胞膜上和细胞质空泡中。在包涵体中没有发现病毒颗粒，尽管这些包涵体中含有免疫荧光证实的病毒抗原。在羧甲基纤维素覆盖下进行的噬菌斑滴定中观察到明显的前带效应。Dugbe病毒感染的PS细胞继续分裂，并建立了慢性感染的培养物，其对同源病毒的重复感染具有抗性。然而，三个披膜病毒，塞姆利基森林，黄热病和乌干达S病毒，和Tahyna病毒，繁殖几乎一样好，在Dugbe载体文化中的控制细胞; Bwamba和内罗毕羊病病毒在Dugbe载体文化的产量降低。有人建议，干扰现象揭示了虫媒病毒的关系，而不是由传统的血清学试验证明。
Dugbe virus multiplied in cell lines of pig and monkey origin producing little gross cytopathogenic effect, but prominent paranuclear, cytoplasmic inclusions were evident in stained preparations examined by light microscopy. Electron microscopic examination of thin sections of Dugbe virus-infected PS cells showed numerous virus particles 90 to 100 nm in diam. on the cell membrane and in cytoplasmic vacuoles. No virus particles were seen in inclusions, although these contained virus antigens demonstrable by immunofluorescence.A marked prozone effect was seen in plaque titrations performed under carboxymethyl cellulose overlay. Dugbe virus-infected PS cells continued to divide and a chronically infected culture was established, which was resistant to superinfection with homologous virus. However, three togaviruses, Semliki Forest, yellow fever and Uganda S viruses, and Tahyna virus, multiplied almost as well in the Dugbe carrier culture as in control cells; Bwamba and Nairobi sheep disease viruses gave reduced yields in the Dugbe carrier cultures. It is suggested that the interference phenomenon reveals arbovirus relationships not demonstrable by conventional serological tests.