Gd-DTPA(2-) as a measure of cartilage degradation
Gd-DTPA(2-) as a measure of cartilage degradation
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DOI：
10.1002/mrm.1910360504
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发表时间：
1996-11-01
影响因子：
3.3
通讯作者：
Burstein, D
中科院分区：
文献类型：
--
作者：
Bashir, A;Gray, ML;Burstein, D
糖胺多聚糖(GAG)是软骨组织固定电荷密度(FCD)的主要来源，在关节炎疾病中早期丢失。我们验证了这样的假设，带电造影剂Gd-DTPA(2-)(因此质子T-1)可以用于测量组织FCD和GAG浓度。软骨外植体的核磁共振波谱研究表明，在Gd-DTPA(2-)存在的情况下，质子T-1与组织钠和GAG浓度有很强的相关性(r&gT；0.96)。考察了理想的一室电化学(Donnan)平衡模型作为从Gd-DTPA(2-)浓度定量FCD的方法，得到的值小于50%，但与已验证的从Na+定量FCD的方法线性相关。这些数据可以作为根据Gd-DTPA(2-)浓度量化FCD的经验模型的基础，或者可以开发更复杂的物理模型。在胰酶和白细胞介素1诱导的软骨降解的T1加权MRI研究中，很容易观察到FCD的空间分布，并具有良好的组织学相关性。因此，Gd-DTPA(2-)中组织的平衡使我们有机会直接成像(通过T-1加权)GAG的浓度，GAG是软骨中的一种主要和至关重要的大分子。初步的临床研究证明Gd-DTPA(2-)可以渗透到软骨中，这表明这项技术在临床上是可行的。
Glycosaminoglycans (GAGs) are the main source of tissue fixed charge density (FCD) in cartilage, and are lost early in arthritic diseases. We tested the hypothesis that, like Na+, the charged contrast agent Gd-DTPA(2-) (and hence proton T-1) could be used to measure tissue FCD and hence GAG concentration, NMR spectroscopy studies of cartilage explants demonstrated that there was a strong correlation (r > 0.96) between proton T-1 in the presence of Gd-DTPA(2-) and tissue sodium and GAG concentrations. An ideal one-compartment electrochemical (Donnan) equilibrium model was examined as a means of quantifying FCD from Gd-DTPA(2-) concentration, yielding a value 50% less but linearly correlated with the validated method of quantifying FCD from Na+. These data could be used as the basis of an empirical model with which to quantify FCD from Gd-DTPA(2-) concentration, or a more sophisticated physical model could be developed. Spatial distributions of FCD were easily observed in T-1-weighted MRI studies of trypsin and interleukin-1 induced cartilage degradation, with good histological correlation. Therefore, equilibration of the tissue in Gd-DTPA(2-) gives us the opportunity to directly image (through T-1 weighting) the concentration of GAG, a major and critically important macromolecule in cartilage. Pilot clinical studies demonstrated Gd-DTPA(2-) penetration into cartilage, suggesting that this technique is clinically feasible.