Syngeneic Murine Metastasis Models: B16 Melanoma
Syngeneic Murine Metastasis Models: B16 Melanoma
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DOI：
10.1007/978-1-4614-8244-4_10
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发表时间：
2014-01-01
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影响因子：
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通讯作者：
Decio, Alessandra
中科院分区：
文献类型：
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作者：
Giavazzi, Raffaella;Decio, Alessandra
小鼠B16黑色素瘤是最常用的肿瘤模型之一，其应用已被用于确定与转移过程和抗癌疗法的发展相关的机制。B16黑色素瘤最初是由Fidler和合作者建立的，作为转移到肺的肿瘤细胞系。从那时起，各种细胞系已衍生，在体外或在体内，具有不同的转移behaviors.The方法用于获得人工转移到肺通过静脉注射B16黑色素瘤细胞和自发转移形成癌细胞生长后的足垫在本章中描述。
The murine B16 melanoma is one of the most used tumor models, its application having been used to determine the mechanisms associated with the metastatic process and the development of anticancer therapies. The B16 melanoma was originally established by Fidler and collaborators as a tumor line metastasizing to the lung. Since that time a variety of cell lines have been derived, in vitro or in vivo, having different metastatic behaviors.The methods used to obtain artificial metastases to the lung through the intravenous injection of B16 melanoma cells and spontaneous metastasis formation following cancer cell growth in the footpad are described in this chapter.