Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective
复制标题
肠道内容物的分子分析：从不同角度阐明拉扎列夫海中同时存在的樽海鞘的摄食
作者：
Katja Metfies;Anja Nicolaus;L. Von Harbou;U. Bathmann;I. Peeken
摘要利用自动核糖体基因间隔区分析(ARSA)和454焦磷酸测序技术对南极盐碱鱼的肠道内容物进行了研究，阐明了南极盐碱鱼的食性。Salp样本是在威德尔海西部的Lazarev海磷虾研究期间(2005-06年夏季和2007-08年夏季、2004年秋季和2006年冬季)收集的。两个盐生物种，Salpa thompsoni和Ihlea racovitzai，都出现在南大洋，可以在地理和季节上重叠。我们提供的证据表明，尽管有非选择性的取食机制，但这两个共存的盐生物种在一个栖息地可能有不同的生态位。93个肠道内容物样本的ARSA图谱显示，即使在相同的采样点，这两个盐生物种之间也存在显著差异。这些差异通过对10个盐类的V4-18S rDNA的454-焦磷酸测序得到了证实。焦磷酸测序数据表明，鞭毛虫，特别是甲藻，构成了两个盐生物种肠道内容物中鉴定的序列读数的很高比例。然而，在甲藻中，两种盐藻的阅读成分存在差异。这支持了之前一项研究的结果，在该研究中，脂肪酸特征表明以鞭毛为基础的饮食，尽管显微镜分析发现硅藻是盐分肠道内容物的主要成分。
Abstract The diet of Antarctic salps was elucidated by investigating their gut content using automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Salp samples were collected during the Lazarev Sea Krill Study in the western Weddell Sea (summer 2005–06 and 2007–08, autumn 2004 and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai, both occur in the Southern Ocean and can overlap geographically and seasonally. We provide evidence that, despite the non-selective feeding mechanism, the two co-occurring salp species might have different niches within a habitat. ARISA-patterns of 93 gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae, constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae, differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate-based diet, even though microscopic analyses identified diatoms as the dominant component of salp gut contents.
影响因子：
1.6
作者：
Wolf C;Frickenhaus S;Kilias ES;Peeken I;Metfies K
通讯作者：
Metfies K