Natural formation of ethylene in forest soils and methods to correct results given by the acetylene-reduction assay
Natural formation of ethylene in forest soils and methods to correct results given by the acetylene-reduction assay
复制标题
DOI：
10.1016/0038-0717(83)90072-x
复制
发表时间：
1983
影响因子：
9.7
通讯作者：
H. Nohrstedt
中科院分区：
文献类型：
--
作者：
H. Nohrstedt
本文研究了三种测定森林土壤中C_2H_4自然形成的方法。(1)在环境空气中与500 μl C2 H2 l − 1孵育，N2酶活性非常低，并强烈抑制了添加的C2 H4的分解。在100 ml C2 H2 l − 1中，C2 H4的自然形成明显低于500 μl l−1.1中。(2)向土壤中添加1 mg g− 1 NH 4 +-N后，在100 ml C2 H2 l − 1中孵育。这种改良剂抑制了N2酶的活性，对C2 H4的自然生成影响不大. (3)在100 ml C2 H2 l − 1和20 ml CO l−1中孵育。该浓度的CO抑制N_2酶活性达95%，但不影响C_2H_4的自然形成，对呼吸作用只有微弱的影响。第一个仅用于检测ILS的发生和可能的意义，另外两个用于确定其确切值。对来自两个针叶林土壤的样品进行测试，第三种方法（C2 H2 + CO）与常规方法（其中对照在环境空气中培养）相比，产生更高的C2 H4天然形成，因此产生更低的N2酶活性。
Three methods to determine the natural formation of C2H4by forest soils were studied in connection with the C2H2-reduction assay. (1) Incubations with 500 μl C2H2l−1in ambient air gave a very low N2ase activity and strongly suppressed the decomposition of added C2H4. The natural formation of C2H4was significantly lower in 100 ml C2H2l−1than in 500 μl l−1.1.(2) Incubations in 100 ml C2H2l−1after amendment of 1 mg g−1NH4+-N to soil. This amendment inhibited N2ase activity, and the natural formation of C2H4was only slightly affected.2.(3) Incubations in 100 ml C2H2l−1with 20ml CO l−1. This concentration of CO suppressed N2ase activity by 95% but did not influence the natural formation of C2H4and had only a weak effect on respiration.It was concluded that the three methods can be used as controls for the natural formation of C2H4in connection with the C2H2-reduction assay, the first only to detect ils occurrence and possible significance and the two other to determine its exact value.Tested on samples from two coniferous forest soils, the third method (C2H2+ CO) gave a higher natural formation of C2H4and consequently a lower N2ase activity than the conventional method in which the control is incubated in ambient air.