Alginate polylysine microcapsules as immune barrier: Permeability of cytokines and immunoglobulins over the capsule membrane
Alginate polylysine microcapsules as immune barrier: Permeability of cytokines and immunoglobulins over the capsule membrane
复制标题
DOI：
10.1016/s0963-6897(97)00002-x
复制
发表时间：
1997-07-01
影响因子：
3.3
通讯作者：
SkjakBraek, G
中科院分区：
文献类型：
--
作者：
Kulseng, B;Thu, B;SkjakBraek, G
海藻酸盐聚赖氨酸微胶囊胰岛移植是治疗I型糖尿病的一种潜在的有效方法，本研究采用一种新方法研究了海藻酸盐聚赖氨酸微胶囊对细胞因子和免疫球蛋白的通透性。磁性单分散聚合物颗粒（Dynabeads）包被针对选定蛋白质的抗体，包被在0.7 mm海藻酸盐聚赖氨酸微胶囊中。胶囊膜对IgG （150 kDa）、转铁蛋白（81 kDa）、肿瘤坏死因子（TNF, 51 kDa）、白细胞介素-1 β (IL-1 β, 17.5 kDa)和胰岛素（5.8 kDa）的通透性通过测量i -125标记的蛋白质与包被抗体包被的Dynabeads的结合来估计。胶囊具有不均匀的固体凝胶核，由高古卢醛酸或高甘露醛酸含量的海藻酸盐和浓度从0.05-0.2%不等的聚l （PLL）或聚d -赖氨酸（PDL）制成。用PLL-而不是pdl -膜制成的胶囊可以穿透转铁蛋白，发现IL-1 β可以穿透所有不同类型的胶囊，然而，高g胶囊可以使TNF不渗透，但仍允许转铁蛋白通过，这些胶囊对IL-1 β的渗透性，但对TNF的渗透性在朗格汉斯小鼠胰岛与TNF和IL-1 β一起培养的实验中得到证实。并比较包被和未包被胰岛的IL-6。(C) 1997爱思唯尔科学有限公司
Transplantation of pancreatic islets in alginate polylysine microcapsules is a potential useful method for treating type I diabetes, In this study, the permeability for alginate-polylysine microcapsules to cytokines an immunoglobulines has been investigated by a newly developed method. Magnetic monodisperse polymer particles (Dynabeads) coated with antibodies against selected proteins were encapsulated in 0.7 mm alginate polylysine microcapsules. The capsule membrane permeability to IgG (150 kDa), Transferrin (81 kDa), Tumor necrosis factor (TNF, 51 kDa), Interleukin-1 beta (IL-1 beta, 17.5 kDa), and insulin (5.8 kDa) was estimated by measuring the binding of I-125-labeled proteins to the encapsulated antibody coated Dynabeads, Capsules with an inhomogeneous solid gel core were made of alginates with high guluronic or high mannuronic acid content and poly-L, (PLL)- or poly-D-lysine (PDL) of concentrations varied from 0.05-0.2%, The various capsules examined were all impermeable to IgG, The capsules made with a PLL-, but not PDL-membranes were permeable for transferrin, IL-1 beta was found to penetrate all of the different capsule types, The high-G capsules, however, could be made impermeable to TNF and still allowed transferrin to pass, The permeability of these capsules to IL-1 beta, but not to TNF was confirmed in an assay where mouse islets of Langerhans were incubated with TNF and IL-1 beta, and comparing the IL-6 for encapsulated and nonencapsulated islets. (C) 1997 Elsevier Science Inc.