Localization and neuronal response of RFamide related peptides in the rat central nervous system
Localization and neuronal response of RFamide related peptides in the rat central nervous system
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DOI：
10.1016/s0006-8993(03)02877-4
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发表时间：
2003-08-29
期刊：
影响因子：
2.9
通讯作者：
Ibata, Y
中科院分区：
文献类型：
--
作者：
Yano, T;Iijima, N;Ibata, Y
RFamide相关肽(RFRP)-1和RFRP-3是来源于同一前蛋白的神经肽。我们使用特异性抗体检测了RFRP-1和RFRP-3免疫反应性（irs）在大鼠中枢神经系统中的分布。在下丘脑尾侧部分、脑室周围核（pern）以及下丘脑腹内侧核周围或上方的部分检测到含有rfrp - 1和RFRP-3的神经元细胞体。免疫组织化学和原位杂交分析显示，含有RFRP免疫反应性和mRNA的神经元与含有神经肽FF的神经元不同，后者在c端含有与RFRP-3相同的结构，即pro - glu - arg - ph - nh2。同时含有RFRP-1和RFRP-3的纤维广泛分布于脑端间隔外侧核、室旁丘脑核、各种下丘脑核、中脑导水管周围灰质、桥脑桥臂旁核和延髓孤束核。仅在脊髓后灰色角内检测到RFRP-1-ir。仅在几个丘脑核和脊髓中检测到rrp -3-ir，特别是在后中间沟和前灰角内。脑室内给予RFRPs可诱导NTS前部、蓝斑核、尾状核、视上核、pern和下丘脑弓状核的c-Fos表达。这些结果表明，RFRP-1和RFRP-3广泛分布于大鼠中枢神经系统，并可能参与神经内分泌系统或疼痛调节等多种功能。(C) 2003 Elsevier B.V.版权所有
RFamide related peptides (RFRP)-1 and RFRP-3 are neuropeptides derived from the same preproprotein. We have examined the distribution of RFRP-1 and RFRP-3 immunoreactivities (irs) in the rat central nervous system using specific antibodies. Neuronal cell bodies containing both RFRP-I and RFRP-3 were detected within the caudal portion of the hypothalamus, the periventricular nucleus (PerVN), and the portion around or above the ventromedial nucleus of the hypothalamus. Both immunohistochemical and in situ hybridization analyses showed that neurons containing RFRP immunoreactivity and mRNA were distinct from those of neuropeptide FF, which contains the same structure at the C-terminus, Pro-Glu-Arg-Phe-NH2, as RFRP-3. Fibers containing both RFRP-1 and RFRP-3 were widely distributed in the brain: the lateral septal nucleus in the telencephalon, the paraventricular thalamic nucleus, various hypothalamic nuclei, the periaqueductal gray in the midbrain, the parabrachial nucleus in the pons, and the nucleus tractus solitarius (NTS) in the medulla oblongata. Only RFRP-1-ir was detected within the posterior gray horn in the spinal cord. Only RFRP-3-ir was detected in several thalamic nuclei and the spinal cord, especially at the posterior intermediate sulcus and within the anterior gray horn. Intracerebroventricular administration of RFRPs induced c-Fos expression in the anterior portion of the NTS, locus coeruleus, the nucleus of incertus, supraoptic nucleus, PerVN and the arcuate nucleus of the hypothalamus. These results show that RFRP-1 and RFRP-3 are widely distributed in the rat central nervous system and might be involved in various functions such as the neuroendocrine system or pain modulation. (C) 2003 Elsevier B.V. All rights reserved.