A heterologous DNA priming-Mycobacterium bovis BCG boosting immunization strategy using mycobacterial Hsp70, Hsp65, and a antigens improves protection against tuberculosis in mice
A heterologous DNA priming-Mycobacterium bovis BCG boosting immunization strategy using mycobacterial Hsp70, Hsp65, and a antigens improves protection against tuberculosis in mice
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DOI：
10.1128/iai.72.12.6945-6950.2004
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发表时间：
2004-12-01
影响因子：
3.1
通讯作者：
Tascon, RE
中科院分区：
文献类型：
--
作者：
Ferraz, JC;Stavropoulos, E;Tascon, RE
结核病每年造成200多万人死亡，全球新病例数量正在上升。DNA疫苗联合卡介苗（BCG）是预防该病的一种潜在策略。在这里，我们使用了一种异源的初免-加强免疫的方法，使用DNA质粒和BCG的组合，以提高疫苗接种对小鼠结核分枝杆菌感染的效力。作为模型抗原，我们选择了M.结核病阿帕（富含丙氨酸-脯氨酸抗原）和免疫显性的Hsp 65和Hsp 70分枝杆菌抗原与BCG组合。我们证明，与单独接种BCG的动物相比，注射表达这些抗原的DNA载体组合的动物在接种BCG时表现出增加的特异性抗分枝杆菌免疫应答。更重要的是，这种方案获得的保护也明显优于单独使用BCG。
Tuberculosis is responsible for >2 million deaths a year, and the number of new cases is rising worldwide. DNA vaccination combined with Mycobacterium bovis bacillus Calmette Guerin (BCG) represents a potential strategy for prevention of this disease. Here, we used a heterologous prime-boost immunization approach using a combination of DNA plasmids and BCG in order to improve the efficacy of vaccination against Mycobacterium tuberculosis infection in mice. As model antigens, we selected the M. tuberculosis Apa (for alanine-proline-rich antigen) and the immunodominant Hsp65 and Hsp70 mycobacterial antigens combined with BCG. We demonstrated that animals injected with a combination of DNA vectors expressing these antigens, when boosted with BCG, showed increased specific antimycobacterial immune responses compared to animals vaccinated with BCG alone. More importantly, the protection achieved with this regimen was also significantly better than with BCG alone.