Cancer stem cell-specific expression profiles reveal emerging bladder cancer biomarkers and identify circRNA_103809 as an important regulator in bladder cancer
Cancer stem cell-specific expression profiles reveal emerging bladder cancer biomarkers and identify circRNA_103809 as an important regulator in bladder cancer
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癌症干细胞特异性表达谱揭示了新兴的膀胱癌生物标志物，并将 circRNA_103809 确定为膀胱癌的重要调节因子
DOI：
10.18632/aging.102816
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发表时间：
2020-02-29
期刊：
影响因子：
5.2
通讯作者：
Wu, Song
中科院分区：
文献类型：
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作者：
Tao, Tao;Yuan, Simin;Wu, Song
膀胱癌干细胞（BCSCs）具有自我更新和分化能力，可能参与膀胱癌的发生、转移、复发和耐药。然而，bscs的潜在功能机制尚不清楚。在本研究中，我们利用膀胱癌患者标本，通过转录组芯片数据分析，描述了BCSCs与膀胱癌非干细胞（BCNSCs）中mrna、lncRNAs和circRNAs的差异表达。CircRNA_103809是在BCSCs中发现的高表达circRNA中排名第一的一个，它作为miR-511海绵，促进膀胱癌的自我更新、迁移和侵袭能力。此外，GO和KEGG通路分析表明，鉴定到的差异表达基因可能参与了癌细胞的细胞代谢、分化和转移调控。WGCNA构建的lncRNAs/ mrna和circRNAs/ mrna共表达网络，揭示了BCSCs中非编码/编码rna的调控模式。值得注意的是，作为网络中的核心基因，AHCY、C6orf136和LRIG1显示出很高的膀胱癌预后潜力。因此，进一步研究BCSCs中非编码RNA的功能机制，对于检测膀胱癌发病机制和发现新的生物标志物具有重要意义。
Bladder cancer stem cells (BCSCs), exhibiting self-renewal and differentiation capacities, may contribute to the tumor initiation, metastasis, recurrence and drug resistance of bladder cancer. However, the underlying functional mechanisms of BCSCs remain to be clarified. In this study, we describe the differentially-expressed mRNAs, lncRNAs, and circRNAs in BCSCs compared with that in bladder cancer non-stem cells (BCNSCs) through the transcriptome microarray data analysis using bladder cancer patients' specimens. CircRNA_103809, the top one among the highly expressed circRNA identified in BCSCs, promotes the self-renewal, migration and invasion capabilities of bladder cancer by acting as a miR-511 sponge. Additionally, GO and KEGG pathway analysis suggest the differentially expressed genes identified may be involved in the cellular metabolism, differentiation and metastasis regulation of the cancer cells. Co-expression networks of lncRNAs/mRNAs and circRNAs/mRNAs constructed by WGCNA give a picture of the non-coding/coding RNAs regulating patterns in BCSCs. Notably, as core genes in the networks, AHCY, C6orf136 and LRIG1 show high potential to be prognosticators for bladder cancer. Therefore, further studies of non-coding RNA functional mechanisms in BCSCs is valuable for detecting the pathogenic mechanisms and discovering novel biomarkers in bladder cancer.