Quantitative analysis of visually induced courtship elements in Drosophila subobscura.
Quantitative analysis of visually induced courtship elements in Drosophila subobscura.
复制标题
果蝇视觉诱导求偶元素的定量分析。
DOI：
10.1080/01677063.2017.1290613
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
D.
中科院分区：
文献类型：
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作者：
Higuchi;T.;Kohatsu;S.;Yamamoto;D.
我们开发了一种新的模式来定量分析苍蝇的求偶行为，FlyMotion-detector with a actuators - coupledstimulator (FlyMacs)，其中用移动的视觉目标刺激苍蝇并记录诱导行为是在计算机控制下自动进行的。我们使用flymac来识别触发亚bobscura果蝇特定求爱元素的运动特征，其交配被认为是强烈依赖于视觉的。雌性的腹部附着在驱动器上，当以适当的模式移动时，在测试中唤起雄性拍打般的前腿运动，中腿摆动和长鼻伸展，这些被认为是雄性求偶行为的基本动作。敲击主要是在目标运动时引起的，而在目标停止运动后最常观察到中腿摆动和长鼻伸展。与通常在靶标停止后（~ 3000 ms）立即发生的腿中部摆动相反，随着靶标停止后时间的推移，喙部伸展的发生率逐渐增加，在3000 ms时达到平稳期。结果表明，轻拍、中腿摆动和长鼻动作都是由视觉目标的不同运动特征引起的。这些发现并不支持单一关键刺激诱发整个求爱仪式的观点。相反，求偶行为与目标的运动和位置相关，这表明d。亚鹦鹉螺通过感官信息来塑造它的求偶模式。
We developed a new paradigm for quantitative analysis of courtship behavior in flies,FlyMotion-detector with anActuator-CoupledStimulator (FlyMacs), in which the stimulation of a fly with a moving visual target and recording of induced behaviors are automated under computer control. We employ FlyMacs for the identification of motion features that trigger specific courtship elements inDrosophila subobscura, whose mating is suggested to be strongly vision dependent. A female abdomen attached to the actuator, when moved in an appropriate pattern, evokes in the test male tapping-like foreleg motions, midleg swing and proboscis extension, which are considered to be elementary actions in male courtship behavior. Tapping is primarily induced when the target is moving, whereas midleg swing and proboscis extension are most frequently observed after the target stops moving. In contrast to midleg swing, which tends to occur immediately after target cessation (∼3000 ms), the incidence of proboscis extension gradually increases with time after target cessation, reaching a plateau at 3000 ms. The results suggest that tapping, midleg swing and proboscis extension are each induced by different movement features of the visual target. These findings do not support the view that a single key stimulus induces the entire courtship ritual. Rather, courtship behaviors inD. subobscuraare correlated with movement and position of the target, which suggests thatD. subobscurauses sensory information to pattern its courtship.