Effect of oestrogen-dependent vasopressin on HPA axis in the median eminence of female rats
Effect of oestrogen-dependent vasopressin on HPA axis in the median eminence of female rats
复制标题
DOI：
10.1038/s41598-019-41714-z
复制
发表时间：
2019-03-26
影响因子：
4.6
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Nishimura, Kazuaki;Yoshino, Kiyoshi;Ueta, Yoichi
正中隆起（ME）在解剖学上由外部（eME）和内部（iME）层组成。下丘脑神经分泌细胞终止于eME中的轴突，并将其调节参与应激反应的垂体前叶激素分泌的神经激素分泌到位于eME中的门静脉中。室旁核（PVN）和视上核（SON）中产生精氨酸加压素（AVP）和催产素的大细胞神经分泌细胞（MNCs）通过iME终止于垂体后叶（PP）的轴突。在这里，我们提供的第一个证据表明，雌激素调节AVP水平的动态变化，在雌性大鼠的eME轴突末梢，使用AVP-eGFP和AVP-DREADDs转基因大鼠。在成年雌性大鼠的发情周期的所有阶段，观察到强烈的AVP-eGFP荧光的eME，但在雄性转基因大鼠。双侧卵巢切除术后，eME中的AVP-eGFP荧光被耗尽，但在高剂量17 β-雌二醇下再次出现。在大多数处理中，MNCs和PP中的AVP-eGFP荧光没有显著变化。外周氯氮平-N-氧化物给药诱导AVP-DREADDs神经元激活，导致转基因大鼠血浆皮质酮水平显著增加。这些结果表明，应激诱导的激活下丘脑-垂体-肾上腺轴可能是由于雌激素依赖性上调雌性大鼠eME中的AVP。
The median eminence (ME) anatomically consists of external (eME) and internal (iME) layers. The hypothalamic neurosecretory cells terminate their axons in the eME and secrete their neurohormones regulating anterior pituitary hormone secretion involved in stress responses into the portal vein located in the eME. Magnocellular neurosecretory cells (MNCs) which produce arginine vasopressin (AVP) and oxytocin in the paraventricular (PVN) and supraoptic nuclei (SON) terminate their axons in the posterior pituitary gland (PP) through the iME. Here, we provide the first evidence that oestrogen modulates the dynamic changes in AVP levels in the eME axon terminals in female rats, using AVP-eGFP and AVP-DREADDs transgenic rats. Strong AVP-eGFP fluorescence in the eME was observed at all oestrus cycle stages in adult female rats but not in male transgenic rats. AVP-eGFP fluorescence in the eME was depleted after bilateral ovariectomy but re-appeared with high-dose 17 beta-oestradiol. AVP-eGFP fluorescence in the MNCs and PP did not change significantly in most treatments. Peripheral clozapine-N-oxide administration induced AVP-DREADDs neurone activation, causing a significant increase in plasma corticosterone levels in the transgenic rats. These results suggest that stress-induced activation of the hypothalamic-pituitary-adrenal axis may be caused by oestrogen-dependent upregulation of AVP in the eME of female rats.