Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F.
Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F.
复制标题
胚胎癌细胞系PCC4-F体外分化细胞的形态学和生化观察。
作者：
A. Chung;L. E. Estes;H. Shinozuka;J. Braginski;C. Lorz;C. Chung
摘要 胚胎癌细胞系 PCC4-F 在体外可分化为多种细胞类型。光学和电子显微镜以及组织化学方法的结合鉴定或强烈表明心肌和骨骼肌、成纤维细胞、纤毛和非纤毛上皮细胞、内胚层卵黄囊细胞、神经源细胞和脂肪细胞的存在。从分化的培养物中分离出两种细胞系。已对这些细胞系与未分化胚胎癌细胞进行了初步比较。衍生的细胞系产生细胞外纤维物质，该物质对高碘酸盐-希夫试剂产生阳性反应，表明糖蛋白性质。当衍生的细胞系与胚胎癌进行比较时，d-[3H]葡萄糖胺和l-[3H]岩藻糖掺入可溶性细胞外蛋白存在显着差异。这些胞外糖蛋白属于狭窄的尺寸类别。酶研究表明存在一种新的乳酸脱氢酶同工酶，这种酶在衍生细胞系中不存在或大大减少。在衍生细胞系中，碱性磷酸酶的比活性显着降低，而酸性磷酸酶、乳酸脱氢酶和葡萄糖-6-磷酸脱氢酶的比活性没有显着差异。衍生系与 Lehman 等人描述的壁内胚层卵黄囊细胞特性的比较。 （J. Cellular Physiol., 84: 13–28, 1974）表明这些新细胞系的特性即使不相同，也非常相似。
Summary The embryonal carcinoma cell line PCC4-F was shown to differentiate in vitro to a variety of cell types. A combination of light and electron microscopy and histochemical methods identifies or strongly suggests the presence of cardiac and skeletal muscle, fibroblasts, ciliated and nonciliated epithelial cells, endodermal yolk sac cells, neurally derived cells, and fat cells. Two cell lines were isolated from differentiated cultures. Preliminary comparisons have been made between these cell lines and the undifferentiated embryonal carcinoma cells. The derived cell lines produce an extracellular fibrous substance that gives a positive reaction to the periodate-Schiff reagent indicating a glycoprotein nature. There are significant differences in the incorporation of d-[3H]glucosamine and l-[3H]fucose into soluble extracellular proteins when the derived cell lines are compared with the embryonal carcinoma. These extracellular glycoproteins fall within narrow size classes. Enzyme studies indicate the presence of a new lactate dehydrogenase isozyme which is absent or greatly diminished in the derived cell lines. The specific activity of alkaline phosphatase is dramatically decreased in the derived cell lines, whereas those of acid phosphatase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase were not significantly different. Comparison of the derived lines with the properties of parietal endodermal yolk sac cells described by Lehman et al. (J. Cellular Physiol., 84: 13–28, 1974) suggests that these new lines are very similar if not identical in properties.