Effects of Enterobacter cloacae on boar sperm quality during liquid storage at 17 °C
Effects of Enterobacter cloacae on boar sperm quality during liquid storage at 17 °C
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DOI：
10.1016/j.anireprosci.2014.05.008
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发表时间：
2014-07-01
影响因子：
2.2
通讯作者：
Yeste, Marc
中科院分区：
文献类型：
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作者：
Prieto-Martinez, Noelia;Bussalleu, Eva;Yeste, Marc
在猪场和人工授精（AI）中心，在精液采集、加工和储存过程中，经常发生新鲜和延长的公猪精子污染。细菌的存在对公猪精子质量产生不利影响，这可能会导致生殖中心的经济损失。本研究首次评估了阴沟肠杆菌的存在如何影响猪精子在15-17 ℃液体储存11天的保存。用不同精菌比（2：1、1：1、1：5和1：10）的E. clothing. 1：0比例（未接种）用作阴性对照。最具感染性的比例（即1：5和1：10）显着损害精子活力和膜的完整性，增加精子凝集，并降低精子的渗透阻力。相比之下，最低细菌浓度（2：1）对公猪精子质量的负面影响明显较低。此外，其他参数如pH值也在最高感染率（即1：5和1：10）下受到更大影响，尽管未观察到精子形态受损。总之，目前的工作表明，E。当细菌浓度高于1：1的比例时，在15-17 ℃的液体储存期间公猪精子中的梭菌会损害精液剂量的寿命和受精能力。进一步的研究是必要的，以解决通过何种机制E。Closectin会损害公猪精子质量。(C)© 2014 Elsevier B. V.保留所有权利。
Contamination of fresh and extended boar sperm often occurs in farms and artificial insemination (AI) centres during semen collection, processing and storage. The presence of bacteria produces detrimental effects on boar sperm quality, which may cause economic losses in reproductive centres. The present study has evaluated for the first time how the presence of Enterobacter cloacae affects the preservation of boar spermatozoa in liquid storage at 15-17 degrees C for an 11-day period. With this purpose, extended semen samples from seven healthy post-pubertal boars were artificially contaminated with different sperm:bacterium ratios (2:1; 1:1; 1:5 and 1:10) of E. cloacae. The 1:0 ratio (non-inoculated) served as a negative control. The most infective ratios (i.e. 1:5 and 1:10) significantly damaged sperm motility and membrane integrity, increased sperm agglutination, and decreased the osmotic resistance of spermatozoa. In contrast, the negative impact that the lowest bacterial concentration (2:1) had on boar sperm quality was clearly lower. In addition, other parameters such as pH were also more affected at the highest infective ratios (i.e. 1:5 and 1:10), despite no damage being observed on sperm morphology. In conclusion, the present work shows that damage inflicted by the presence of E. cloacae in boar sperm during liquid storage at 15-17 degrees C compromises the longevity and fertilising ability of seminal doses when bacterial concentration is higher than a 1:1 ratio. Further research is warranted to address by which mechanism E. cloacae impairs boar sperm quality. (C) 2014 Elsevier B.V. All rights reserved.