Neurons are generated in confluent astroglial cultures of rat neonatal neocortex
Neurons are generated in confluent astroglial cultures of rat neonatal neocortex
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DOI：
10.1016/s0306-4522(96)00676-8
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发表时间：
1997-06-01
期刊：
影响因子：
3.3
通讯作者：
Meyer, DK
中科院分区：
文献类型：
--
作者：
Hildebrand, B;Olenik, C;Meyer, DK
端脑的细胞在特定的增殖区产生，神经元和神经胶质前体从增殖区迁移到目的地。最近的证据表明，一些前体细胞不会变成分化的细胞，但保持其增殖能力。在这里，我们报告说，神经元可以起源于原代培养的星形胶质细胞从新生大鼠的新皮层。第一个神经元细胞在汇合前不久出现，此时胶质细胞单层正在形成。汇合后，这些仍未分化的细胞数量增加。后来，他们成为神经元特异性标记微管相关蛋白2a，b的免疫化学阳性。它们还含有神经毒素-I蛋白以及编码神经毒素-H的特异性信使RNA。观察到它们摄取溴脱氧尿苷表明它们合成DNA，即它们增殖。当用胎牛血清代替Dulbecco改良的基本培养基时，神经元的出现取决于分散细胞的接种密度。这不再是这样的情况下，当培养物保持在Dulbecco的改良的基本培养基/F12 medium，其中转铁蛋白，胰岛素和氯化硒已added.It的结论是，神经元前体细胞可以在原代星形胶质细胞的文化生存。在星形胶质细胞汇合后，如果存在适当的条件，则前体细胞增殖。我们的观察为研究培养的神经元和神经元-胶质细胞相互作用提供了一个新的模型。(C)1997年IBRO。爱思唯尔科技有限公司出版
Cells of the telencephalon are generated in specific proliferative zones from which neuronal and glial precursors migrate to their destinations. Recent evidence indicates that some precursors do not turn into differentiated cells but keep their ability to proliferate. Here, we report that neurons can originate in primary cultures of astroglial cells prepared from neocortex of newborn rats. The first neuronal cells appeared shortly before confluence, when a glial monolayer was being formed. After confluence, these still undifferentiated cells increased in number. Later, they became immunohistochemically positive for the neuron-specific marker microtubule-associated protein 2a,b. They also contained neurofilament-l protein as well as the specific messenger RNA coding for neurofilament-H. The observation that they took up bromo-deoxyuridine indicated that they synthesized DNA, i.e. they proliferated. When Dulbecco's modified essential medium was substituted with fetal calf serum, the appearance of neurons depended on the seeding density of the dispersed cells. This was no longer the case, when the cultures were maintained in Dulbecco's modified essential medium/F12 medium to which transferrin, insulin and selenium chloride had been added.It is concluded that neuronal precursors can survive in primary astroglial cultures. After confluence of the astroglial cells the precursors proliferate if appropriate conditions are present. Our observation provides a new model for the investigation of cultured neurons and neuronal-glial interactions. (C) 1997 IBRO. Published by Elsevier Science Ltd.