Digital imaging analysis for the study of endotoxin-induced mitochondrial ultrastructure injury.
Digital imaging analysis for the study of endotoxin-induced mitochondrial ultrastructure injury.
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用于研究内毒素诱导的线粒体超微结构损伤的数字成像分析。
DOI：
10.1155/2000/201406
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Bauer JA
中科院分区：
文献类型：
--
作者：
Joshi MS;Crouser ED;Julian MW;Schanbacher BL;Bauer JA
线粒体功能的原发性缺陷与100多种疾病有关。在原位，线粒体在正常健康细胞中具有独特和明确的形态，但与线粒体功能缺陷相关的疾病的特征在于存在形态异常和肿胀的线粒体，其嵴扭曲。线粒体形态学的原位研究被确立为线粒体健康的指标，但迄今为止，评估一直是通过受过训练的观察员使用不连续评分系统的主观评价。在这里，我们研究了数字成像分析的价值，以提供无偏的，可重复的，方便的线粒体超微结构的评价。回肠粘膜线粒体的电子显微镜照片进行了研究，使用我们以前描述的评分系统，并通过使用六个数字参数，定义的大小，形状和电子密度超过700个单独的线粒体的特征进行数字化分析。使用主观评分系统和数字成像参数，在LPS处理的动物中检测到线粒体形态相对于载体对照的统计学显著变化（p < 0：05）。然而，成像方法提供了方便和高通量的能力，并且很容易自动化，以消除研究者的影响。这些结果说明了脓毒症期间回肠粘膜线粒体超微结构的显着变化，并证明了数字成像技术在这种情况下进行常规评估的价值。
Primary defects in mitochondrial function have been implicated in over 100 diverse diseases. In situ, mitochondria possess unique and well-defined morphology in normal healthy cells, but diseases linked to defective mitochondrial function are characterized by the presence of morphologically abnormal and swollen mitochondria with distorted cristae. In situ study of mitochondrial morphology is established as an indicator of mitochondrial health but thus far assessments have been via subjective evaluations by trained observers using discontinuous scoring systems. Here we investigated the value of digital imaging analysis to provide for unbiased, reproducible, and convenient evaluations of mitochondrial ultrastructure. Electron photomicrographs of ileal mucosal mitochondria were investigated using a scoring system previously described by us, and also analyzed digitally by using six digital parameters which define size, shape, and electron density characteristics of over 700 individual mitochondria. Statistically significant changes in mitochondrial morphology were detected in LPS treated animals relative to vehicle control using both the subjective scoring system and digital imaging parameters (p < 0:05). However, the imaging approach provided convenient and high throughput capabilities and was easily automated to remove investigator influences. These results illustrate significant changes in ileal mucosal mitochondrial ultrastructure during sepsis and demonstrate the value of digital imaging technology for routine assessments in this setting.