Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables.
Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables.
复制标题
用于水果和蔬菜采后农药测定的化学计量辅助 QuEChERS 提取方法。
DOI：
10.1038/srep42489
复制
发表时间：
2017-02-22
影响因子：
4.6
通讯作者：
Fan B
中科院分区：
文献类型：
--
作者：
Li M;Dai C;Wang F;Kong Z;He Y;Huang YT;Fan B
建立了一种基于化学计量学工具的水果和蔬菜中5种不同农药（2，4-二氯苯氧乙酸（2，4-D）、多菌灵、噻菌灵、异菌脲和咪鲜胺）的同时定量分析方法。在改进的QuEChERS（quick，easy，cheap，effective，rugged and safe）方法中，采用Plackett-Burman（P-B）筛选设计，比较了优化提取和净化分析的因素和响应。采用中心组合设计（CCD）结合Derringer期望函数（DF）对影响因素甲苯含量、盐酸含量和石墨化炭黑（GCB）用量进行了优化。估计食品基质中2，4-D、多菌灵、噻菌灵和咪鲜胺的定量限（LOQ）为1.0 μg/kg，异菌脲为1.5 μg/kg。在3个加标水平下，平均回收率在70.4-113.9%范围内，相对标准偏差（RSD）小于16.9%。采用自下而上的方法测定分析方法的测量不确定度，平均值为7.6%。多菌灵是最常见的真实的样品分析使用开发的方法。因此，该分析方法可以作为一个有利的和快速的工具，用于水果和蔬菜中的5种防腐剂农药的测定。
An effective analysis method was developed based on a chemometric tool for the simultaneous quantification of five different post-harvest pesticides (2,4-dichlorophenoxyacetic acid (2,4-D), carbendazim, thiabendazole, iprodione, and prochloraz) in fruits and vegetables. In the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, the factors and responses for optimization of the extraction and cleanup analyses were compared using the Plackett–Burman (P–B) screening design. Furthermore, the significant factors (toluene percentage, hydrochloric acid (HCl) percentage, and graphitized carbon black (GCB) amount) were optimized using a central composite design (CCD) combined with Derringer’s desirability function (DF). The limits of quantification (LOQs) were estimated to be 1.0 μg/kg for 2,4-D, carbendazim, thiabendazole, and prochloraz, and 1.5 μg/kg for iprodione in food matrices. The mean recoveries were in the range of 70.4–113.9% with relative standard deviations (RSDs) of less than 16.9% at three spiking levels. The measurement uncertainty of the analytical method was determined using the bottom-up approach, which yielded an average value of 7.6%. Carbendazim was most frequently found in real samples analyzed using the developed method. Consequently, the analytical method can serve as an advantageous and rapid tool for determination of five preservative pesticides in fruits and vegetables.