Synthesis and immunological study of a wall teichoic acid-based vaccine against E-faecium U0317
Synthesis and immunological study of a wall teichoic acid-based vaccine against E-faecium U0317
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壁磷壁酸疫苗的合成及免疫学研究
DOI：
10.1080/07328303.2017.1390576
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发表时间：
2017
影响因子：
1
通讯作者：
Wu Zhimeng
中科院分区：
文献类型：
--
作者：
Zhou Zhifang;Ding Wenzhang;Li Chen;Wu Zhimeng
从大肠杆菌中分离出的细胞壁磷壁酸 (WTA) 重复单元。通过分步策略有效地化学合成了faeciumU0317。它用 5-氨基戊基接头衍生化，以促进与载体蛋白 KLH 和 HSA 的缀合。 KLH 缀合物的免疫学研究1表明，它可以激发强大的免疫反应和高滴度的IgG抗体，从而可以成功识别合成的WTA重复单元3。这一结果表明，合成糖缀合物 1 可能是一种有前途的抗大肠杆菌疫苗候选物。粪便用于进一步研究。
A repeat unit of cell wall teichoic acids (WTA) isolated fromE. faeciumU0317 was chemically synthesized efficiently by a stepwise strategy. It was derivatized with a 5-aminopentanyl linker to facilitate conjugation with carrier proteins KLH and HSA. Immunological studies of the KLH conjugate1demonstrated that it could provoke robust immune responses and high titers of IgG antibodies, which could successfully recognize the synthesized WTA repeat unit3. This result suggested that synthetic glycoconjugate1could be a promising vaccine candidate againstE. faeciumfor further studies.