The effects of different high-fat (lard, soybean oil, corn oil or olive oil) diets supplemented with fructo-oligosaccharides on colonic alkaline phosphatase activity in rats
The effects of different high-fat (lard, soybean oil, corn oil or olive oil) diets supplemented with fructo-oligosaccharides on colonic alkaline phosphatase activity in rats
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DOI：
10.1007/s00394-020-02219-y
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发表时间：
2020-03
影响因子：
5
通讯作者：
Y. Okazaki;T. Katayama
中科院分区：
文献类型：
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作者：
Y. Okazaki;T. Katayama
目的我们最近报道，在高脂（HF）饮食的大鼠中，包括低聚果糖（FOS）在内的可发酵的不易消化的碳水化合物通常会提高结肠碱性磷酸酶（ALP）的活性和IAP-I（一种ALP基因）的表达，并且还会提高肠道粘蛋白并调节肠道微生物群。本研究旨在探讨膳食脂肪类型是否会影响低聚果糖对高频喂养大鼠结肠 ALP 活性和管腔环境的影响。方法给雄性 Sprague-Dawley 大鼠喂食含有 30% 大豆油、玉米油、橄榄油或猪油（含或不含 4% 低聚果糖）的饮食 2 周。测量了结肠 ALP 活性、基因表达和肠腔变量（包括粘蛋白和微生物群）。结果在猪油饮食组中，膳食低聚果糖显着提高了结肠 ALP 活性和 IAP-I 的表达。在橄榄油饮食组中也观察到低聚果糖对结肠 ALP 活性的升高作用，尽管这里 IAP-I 表达没有改变。然而，大豆油和玉米油饮食组没有表现出低聚果糖对结肠碱性磷酸酶的升高作用。无论膳食脂肪类型如何，膳食低聚果糖都能显着提高粪便 ALP 和粘蛋白，并且低聚果糖的效果在猪油饮食组中更为突出。在猪油和橄榄油饮食组中，粪便中观察到的乳酸菌数量因低聚果糖而显着增加，但在豆油和玉米油饮食组中则没有。结论本研究表明，膳食脂肪类型可能会改变低聚果糖对结肠腔环境的影响，包括高脂饮食大鼠的 ALP 活性。
PurposeWe recently reported that fermentable non-digestible carbohydrates including fructo-oligosaccharides (FOS) commonly elevate colonic alkaline phosphatase (ALP) activity and the expression ofIAP-I, an ALP gene, in rats fed a high-fat (HF) diet, and also elevate gut mucins and modulate gut microbiota. This study aims to investigate whether dietary fat types influence the effect of FOS on colonic ALP activity and the luminal environment in HF-fed rats.MethodsMale Sprague–Dawley rats were fed a diet containing 30% soybean oil, corn oil, olive oil or lard with or without 4% FOS for 2 weeks. Colon ALP activity, gene expression, and gut luminal variables including mucins and microbiota were measured.ResultsIn the lard diet groups, dietary FOS significantly elevated colonic ALP activity and the expression ofIAP-I. The elevating effect of FOS on colonic ALP activity was also observed in the olive oil diet groups, although here theIAP-Iexpression was not changed. However, the soybean oil and corn oil diet groups did not exhibit the elevating effect of FOS on colon ALP. Fecal ALP and mucins were significantly elevated by dietary FOS regardless of dietary fat types, and the effect of FOS was prominent in the lard diet groups. The number ofLactobacillusspp.observed in fecal matter was significantly increased by dietary FOS in the lard and olive oil diet groups, but not in the soybean oil and corn oil diets groups.ConclusionThis study suggests that dietary fat types may change the effect of FOS on the colonic luminal environment including the ALP activity in rats fed a high-fat diet.