Pain exacerbates chronic mild stress-induced changes in noradrenergic transmission in rats
Pain exacerbates chronic mild stress-induced changes in noradrenergic transmission in rats
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DOI：
10.1016/j.euroneuro.2014.01.011
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发表时间：
2014-06-01
影响因子：
5.6
通讯作者：
Berrocoso, Esther
中科院分区：
文献类型：
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作者：
Bravo, Lidia;Torres-Sanchez, Sonia;Berrocoso, Esther
抑郁症可以影响疼痛，反之亦然，但一种疾病如何影响另一种疾病的病理生理学的生物学机制仍不清楚。蓝斑-去甲肾上腺素能传递的失调与这两种情况有关，尽管尚不清楚这种影响是否在共病抑郁症和慢性疼痛的情况下加剧。我们研究了蓝斑活动，使用免疫荧光和电生理方法在大鼠进行不可预测的慢性轻度应激（CMS，抑郁症的实验模型）和/或慢性压迫性损伤（CCI，慢性神经性疼痛的模型）2周。CCI单独没有任何影响的蓝斑参数的研究，而CMS导致蓝斑的电生理活动略有减少。此外，CMS与蓝斑中酪氨酸羟化酶阳性细胞数量的增加有关，尽管它们的大小较小。有趣的是，CMS的这些作用在与CCI联合时加剧，即使在任何组中均未观察到α 2-肾上腺素受体或去甲肾上腺素转运蛋白的变化。总之，这些研究结果表明，CMS触发蓝斑-去甲肾上腺素能传递的几个修改，这些修改由共病慢性疼痛加剧。(C)2014 Elsevier B.V.和ECNP。All rights reserved.
Depression can influence pain and vice versa, yet the biological mechanisms underlying how one influences the pathophysiology of the other remains unclear., Dysregulation of locus coeruleus-noradrenergic transmission is implicated in both conditions, although it is not known whether this effect is exacerbated in cases of co-morbid depression and chronic pain. We studied locus coeruleus activity using immunofluorescence and electrophysiological approaches in rats subjected to unpredictable chronic mild stress (CMS, an experimental model of depression) and/or chronic constriction injury (CCI, a model of chronic neuropathic pain) for 2 weeks. CCI alone had no effect on any of the locus coeruleus parameters studied, while CMS led to a slight reduction in the electrophysiological activity of the locus coeruleus. Furthermore, CMS was associated with an increase in the number of tyrosine hydroxylase-positive cells in the locus coeruleus, although they were smaller in size. Interestingly, these effects of CMS were exacerbated when combined with CCI, even though no changes in the alpha 2-adrenoreceptors or the noradrenaline transporter were observed in any group. Together, these findings suggest that CMS triggers several modifications in locus coeruleus-noradrenergic transmission that are exacerbated by co-morbid chronic pain. (C) 2014 Elsevier B.V. and ECNP. All rights reserved.