A lymphocyte-specific CC chemokine, secondary lymphoid tissue chemokine (SLC), is a highly efficient chemoattractant for B cells and activated T cells
A lymphocyte-specific CC chemokine, secondary lymphoid tissue chemokine (SLC), is a highly efficient chemoattractant for B cells and activated T cells
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DOI：
10.1002/(sici)1521-4141(199805)28:05
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发表时间：
1998-05-01
影响因子：
5.4
通讯作者：
Yoshie, O
中科院分区：
文献类型：
--
作者：
Nagira, M;Imai, T;Yoshie, O
次级淋巴组织趋化因子（SLC）是主要在淋巴结、阑尾和脾中表达的CC趋化因子，并且对淋巴细胞具有特异性趋化性（Nagira等人，J. Biol. Chem. 1997. 272：19518-19524）。在这里，我们进行了跨内皮迁移试验，以确定类和淋巴细胞向SLC迁移的子集。SLC以高效率吸引新鲜分离的B细胞，并适度吸引T细胞。因此，SLC是第一个对新鲜B细胞具有强趋化活性的CC趋化因子。在T细胞类型和亚群中，SLC广泛吸引CD 4（+）和CD 8（+）细胞、CD 45 RO（-）（初始）和CD 45 RO（+）（记忆）细胞以及CD 26（高）（活化）和CD 26（低/-）（静息）细胞。SLC还吸引各种T细胞亚群和B细胞的L-选择素（+）和L-选择素（-）亚群。此外，促有丝分裂刺激强烈增强T细胞和B细胞向SLC的迁移反应。通过原位杂交，SLC mRNA在淋巴结和阑尾的皮质滤泡旁区域（T细胞区域）中被检测到。总的来说，SLC可能是一种基本的趋化因子，支持广谱淋巴细胞向次级淋巴组织的稳态迁移。SLC还可能通过在抗原刺激后诱导T和B细胞的高效迁移而参与免疫应答。
Secondary lymphoid tissue chemokine (SLC) is a CC chemokine expressed mainly in lymph nodes, appendix and spleen, and specifically chemotactic for lymphocytes (Nagira et at., J. Biol. Chem. 1997. 272: 19518-19524). Here, we carried out transendothelial migration assays to determine the classes and subsets of lymphocytes migrating toward SLC. SLC attracted freshly isolated B cells with high efficiency and T cells modestly. Thus, SLC is the first CC chemokine with a strong chemotactic activity on fresh B cells. Among T cell types and subsets, SLC broadly attracted CD4(+) and CD8(+) cells, CD45RO(-) (naive) and CD45RO(+) (memory) cells, and CD26(high) (activated) and CD26(low/-)(resting) cells. SLC also attracted both L-selectin(+) and L-selectin(-) subpopulations of various T cell subsets and B cells. Furthermore, mitogenic stimulation strongly enhanced migratory responses of T cells and B cells toward SLC. By in situ hybridization, SLC mRNA was detected in the cortical parafollicular regions (the T cell areas) of a lymph node and an appendix. Collectively, SLC may be a basic chemokine supporting homeostatic migration of a broad spectrum of lymphocytes into the secondary lymphoid tissues. SLC may also be involved in immune responses by inducing highly efficient migration of T and B cells following antigenic stimulation.