Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina.
Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina.
复制标题
颈上神经节切除术诱导大鼠视网膜中生长因子表达的变化。
DOI：
10.1167/iovs.05-0656
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Steinle,JenaJ
中科院分区：
文献类型：
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作者：
Wiley,LukeA;Berkowitz,BruceA;Steinle,JenaJ
methods. Surgical sympathectomy (SNX) was used to remove sympathetic innervation to the eye. Real-time PCR was used to measure steady state mRNA expression of VEGF, VEGFR-2, angiopoietin-1, and Tie2. Western blot analysis was performed to assess protein expression. Blood–retinal barrier (BRB) permeability surface area product (PS) was measured using enhanced MRI on a separate group of control and SNX rats.results. mRNA of both VEGF and VEGFR-2 decreased significantly at 6 weeks after SNX. VEGF protein expression also decreased significantly. VEGFR-2 protein was unchanged. Both angiopoietin-1 and Tie2 mRNA expression increased significantly after SNX. Immunoblot analysis showed that angiopoietin-1 protein expression coincided with its mRNA expression. Tie2 protein expression was unaffected. Sympathetic denervation did not significantly increase BRB PS.conclusions. Surgical SNX results in a reduction in VEGF gene and protein expression, while increasing protein expression of Ang-1. These findings suggest that sympathetic nerves regulate the expression of angiogenic growth factors in rat retina.
