Evidence for in vivo thermosensitivity of serotonergic neurons in the rat dorsal raphe nucleus and raphe pallidus nucleus implicated in thermoregulatory cooling
Evidence for in vivo thermosensitivity of serotonergic neurons in the rat dorsal raphe nucleus and raphe pallidus nucleus implicated in thermoregulatory cooling
复制标题
DOI：
10.1016/j.expneurol.2010.11.012
复制
发表时间：
2011-02-01
影响因子：
5.3
通讯作者：
Lowry, Christopher A.
中科院分区：
文献类型：
--
作者：
Hale, Matthew W.;Dady, Kathleen F.;Lowry, Christopher A.
感知环境温度和体温升高并做出适当反应的能力对所有动物的生存至关重要。虽然有证据表明，大脑中的多巴胺能系统在体温调节中发挥作用，包括体温调节冷却，但在体内，在未麻醉的动物中，在热暴露期间，缺乏脑干多巴胺能神经元激活的证据。在这个实验中，我们测试了这一假设，即中脑和延髓中缝复合体中的多巴胺能神经元群体在暴露于温暖的环境温度后被激活。大鼠暴露于温育室在温暖的环境温度（37摄氏度）或室温（RT; 23摄氏度）为105分钟。大脑，然后被删除和处理的立即早期基因c-fos（作为神经元激活的标志物）和色氨酸羟化酶（作为标记的多巴胺能神经元）的蛋白质产物的免疫组织化学检测。暴露于温暖的环境温度增加体温和c-Fos的表达在地形组织群体中缝背核的多巴胺能神经元。中缝背核多巴胺能系统的激活与暴露于孵育室后的体温呈正相关。在延髓，暴露于温暖的环境温度，与暴露于RT相比，减少c-Fos的表达在中缝苍白核的多巴胺能神经元和非多巴胺能细胞的头端腹外侧延髓。总之，这些结果提供了证据，多个但解剖学离散的温度敏感性神经元能系统，可能有影响的体温调节，以及通过预测前脑目标，认知和情感功能。(C)2010年爱思唯尔公司All rights reserved.
The ability to sense and respond appropriately to increases in ambient and body temperatures is critical for the survival of all animals. Although evidence suggests that brain serotonergic systems play a role in thermoregulation, including thermoregulatory cooling, evidence for activation of brainstem serotonergic neurons in vivo, in unanesthetized animals, during heat exposure is lacking. In this experiment we tested the hypothesis that populations of serotonergic neurons in the midbrain and medullary raphe complex are activated following exposure to warm ambient temperature. Rats were exposed to an incubation chamber at either warm ambient temperature (37 degrees C) or room temperature (RT; 23 degrees C) for 105 min. Brains then were removed and processed for immunohistochemical detection of the protein product of the immediate-early gene c-fos (as a marker of neuronal activation) and tryptophan hydroxylase (as a marker of serotonergic neurons). Exposure to warm ambient temperature increased body temperature and c-Fos expression in topographically organized populations of serotonergic neurons in the dorsal raphe nucleus. Activation of the dorsal raphe nucleus serotonergic system was positively correlated with body temperature following exposure to the incubation chamber. In the medulla, exposure to warm ambient temperature, compared with exposure to RT, decreased c-Fos expression in serotonergic neurons in the raphe pallidus nucleus and in non-serotonergic cells in the rostral ventrolateral medulla. Together, these results provide evidence for multiple but anatomically discrete thermosensitive serotonergic systems that may have implications for the regulation of body temperature, as well as, via projections to forebrain targets, cognitive and affective functions. (C) 2010 Elsevier Inc. All rights reserved.