Reduced invasion and growth of Plasmodium falciparum into elliptocytic red blood cells with a combined deficiency of protein 4.1, glycophorin C, and p55
Reduced invasion and growth of Plasmodium falciparum into elliptocytic red blood cells with a combined deficiency of protein 4.1, glycophorin C, and p55
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DOI：
10.1182/blood.v87.8.3462.bloodjournal8783462
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发表时间：
1996-04-15
期刊：
影响因子：
20.3
通讯作者：
Liu, SC
中科院分区：
文献类型：
--
作者：
Chishti, AH;Palek, J;Liu, SC
在这项调查中，我们测量了恶性疟原虫的侵入和生长的椭圆红细胞（RBC）从纯合子遗传性椭圆红细胞增多症的受试者。这些椭圆红细胞先前已被表征为具有蛋白4.1和血型糖蛋白C的分子缺陷。我们的研究结果表明，恶性疟原虫侵入这些蛋白4.1（-）红细胞显着减少。血型糖蛋白C（-）Leach RBC在体外对寄生虫入侵具有相似的抗性。侵入蛋白4.1（-）RBC的寄生虫的细胞内发育也显著减少。相反，在血型糖蛋白C（-）Leach RBC中没有观察到细胞内寄生虫生长的减少。结合我们最近发现的第三种蛋白质p55也缺乏蛋白4.1（-）和血型糖蛋白C（-）红细胞，目前的数据强调了膜相关的蛋白4.1，血型糖蛋白C和p55之间的三元复合物的重要性，在疟疾寄生虫入侵和生长到人类红细胞。(C)1996年，美国血液学会。
In this investigation, we have measured the invasion and growth of the malaria parasite Plasmodium falciparum into elliptocytic red blood cells (RBCs) obtained from subjects with homozygous hereditary elliptocytosis. These elliptocytic RBCs have been previously characterized to possess molecular defects in protein 4.1 and glycophorin C. Our results show that the invasion of Plasmodium falciparum into these protein 4.1 (-) RBCs is significantly reduced. Glycophorin C (-) Leach RBCs were similarly resistant to parasite invasion in vitro. The intracellular development of parasites that invaded protein 4.1 (-) RBCs was also dramatically reduced. In contrast, no such reduction of intracellular parasite growth was observed in the glycophorin C (-) Leach RBCs. In conjunction with our recent finding that a third protein termed p55 is also deficient in protein 4.1 (-) and glycophorin C (-) RBCs, the present data underscore the importance of the membrane-associated ternary complex between protein 4.1, glycophorin C, and p55 during the invasion and growth of malaria parasites into human RBCs. (C) 1996 by The American Society of Hematology.