Distribution of T cells bearing different forms of the T cell receptor gamma/delta in normal and pathological human tissues.
Distribution of T cells bearing different forms of the T cell receptor gamma/delta in normal and pathological human tissues.
复制标题
携带不同形式 T 细胞受体 γ/δ 的 T 细胞在正常和病理人体组织中的分布。
作者：
B. Falini;L. Flenghi;S. Pileri;P. Pelicci;M. Fagioli;M. Martelli;L. Moretta;E. Ciccone
用apap技术对正常和病理人体组织的冷冻切片进行免疫染色，用3个针对TCR γ δ不同表位的单抗进行免疫染色；TCR δ 1与所有携带TCR δ的细胞结合；通过免疫沉淀研究，BB3和δ TCS1似乎分别与TCR δ的二硫连接和非二硫连接形式发生反应。在正常胸腺中，TCR δ 1+细胞约占CD3+胸腺细胞的2%，髓质中TCR δ 1+细胞的数量约为皮层的3倍。TCR δ 1+细胞主要由δ TCS1反应亚群构成（δ TCS1/BB3平均比值：3.7）。在扁桃体中，TCR δ 1+细胞（约占CD3+元素的3%）主要位于滤泡间区，它们经常倾向于排列在高内皮小静脉周围。在大多数样本中，TCR δ 1+细胞分布在扁桃体上皮下。与胸腺不同，大多数TCR δ 1+细胞通常由BB3反应亚群组成（平均BB3/ δ TCS1比值：2.0）。在正常外周血中也观察到类似的BB3+优于δ TCS1+细胞。脾脏是TCR δ 1+细胞浓度最高的器官，与胸腺一样，以δ TCS1+元素为主。值得注意的是，TCR δ 1+细胞优先位于脾窦，而携带TCR α β的淋巴细胞主要位于青霉菌动脉的小动脉周围鞘。研究的大多数肿瘤T细胞增殖缺乏表达TCR γ δ。然而，2例β F1-(TCR α β -) T淋巴母细胞淋巴瘤为TCR γ δ + (δ TCS1+/BB3-)。两者均表现为II期皮质表型，如CD1+/CD3+/CD4+/CD8+/TCR δ 1+。在炎症条件下，在菊地病和结核性淋巴结炎的病例中，观察到BB3+细胞的增加与坏死区域密切相关。这一发现的意义正在研究中。
Frozen sections from normal and pathologic human tissues were immunostained by the APAAP technique with three mAb directed against different epitopes of the TCR gamma delta; TCR delta 1 which binds to all cells bearing the TCR gamma delta; BB3 and delta TCS1 which, by immunoprecipitation studies, appear to react respectively with the disulfide-linked and nondisulfide-linked form of the TCR gamma delta. In normal thymus, TCR delta 1+ cells accounted for approximately 2% of the CD3+ thymocytes and were about three times more numerous in the medulla than in the cortex. TCR delta 1+ cells were mostly constituted by the delta TCS1 reactive subset (average ratio delta TCS1/BB3: 3.7). In the tonsil, the TCR delta 1+ cells (about 3% of CD3+ elements) were mainly located in the interfollicular area, where they frequently tended to arrange around high endothelium venules. In most samples, TCR delta 1+ cells were distributed beneath to the tonsil epithelium. Unlike thymus, the majority of TCR delta 1+ cells were usually constituted by the BB3-reactive subset (average BB3/delta TCS1 ratio: 2.0). A similar predominance of BB3+ over delta TCS1+ cells was also observed in normal peripheral blood. The spleen was the organ with the highest concentration of TCR delta 1+ cells that, like in the thymus, were mostly represented by delta TCS1+ elements. Noteworthy, the TCR delta 1+ cells were preferentially located in the splenic sinusoids while TCR alpha beta-bearing lymphocytes mostly occupied the periarteriolar sheaths of penicilliary arteries. The majority of neoplastic T cell proliferations studied lacked to express the TCR gamma delta. Two cases of beta F1-(TCR alpha beta-) T lymphoblastic lymphoma, however, were TCR gamma delta+ (delta TCS1+/BB3-). Both of them showed a stage II cortical phenotype, e.g., CD1+/CD3+/CD4+/CD8+/TCR delta 1+. Among inflammatory conditions, an increase of BB3+ cells was observed in close association with necrotic areas in cases of Kikuchi's and tuberculous lymphadenitis. The significance of this finding is under study.