Flow dependence of transtubular potential difference in isolated perfused segments of rabbit proximal convoluted tubule.
Flow dependence of transtubular potential difference in isolated perfused segments of rabbit proximal convoluted tubule.
复制标题
兔近曲小管分离灌注段跨管电位差的流量依赖性。
DOI：
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发表时间：
1971
影响因子：
15.9
通讯作者：
F. Rector
中科院分区：
文献类型：
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作者：
J. Kokko;F. Rector
在离体灌流的家兔近曲小管上研究了跨膜电位差（pd）。在灌注流速高于10 nl/min时，当用与浴相同的兔血清的等渗超滤液灌注时，pd为-5.80 +/-0.3 mv（管腔阴性）。这种pd是由小管的转运活性产生的，这一点得到了三个独立观察结果的支持：（a）pd从37 ℃冷却到25 ℃时可逆地降低;（B）当向浴中加入10（-5）M哇巴因时，pd降低，当除去哇巴因时，pd恢复到对照水平;（c）加热到47 ℃时，pd不可逆地降低到零。PD的幅度与灌注流速相关，流速低于10 nl/min。流速的降低与PD的降低相关。排除了管的几何形状和跨膜静水压力作为介导的因素，管理所观察到的pd的大小。
Transmembrane potential difference (pd) was studied in isolated perfused segments of rabbit proximal convoluted tubules. At perfusion flow rates above 10 nl/min the pd was -5.80 +/-0.3 mv (lumen negative) when perfusing with isosmolal ultrafiltrate of same rabbit serum as the bath. That this pd is generated by transport activity of the tubule is supported by three separate observations: (a) pd reversibly decreased with cooling from 37 degrees C to 25 degrees C; (b) pd decreased when 10(-5) M ouabain was added to the bath and reversed to control levels when ouabain was removed; and (c) heating to 47 degrees C irreversibly decreased pd to zero. The magnitude of the pd was related to perfusion flow rate at slower rates than 10 nl/min. A decrease in flow rate was associated with a decrease in pd. The tubular geometry and transmembrane hydrostatic pressure were ruled out as the mediating factors governing the magnitude of observed pd.