Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex
Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex
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DOI：
10.1242/dev.152611
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发表时间：
2017-10-15
期刊：
影响因子：
4.6
通讯作者：
Yang, Jing
中科院分区：
文献类型：
--
作者：
Aguero, Tristan;Jin, Zhigang;Yang, Jing
在发育中的胚胎中，原始生殖细胞（PGCs）代表配子的唯一祖细胞，它们的丢失导致成年不育。在早期发育过程中，PGCs暴露于许多指定体细胞命运的信号。为了防止体细胞分化，PGCs必须暂时沉默它们的基因组，这是一个需要Nanos活性的早期发育过程。然而，目前还不清楚Nanos翻译在发育中的胚胎中是如何调节的。我们在这里报告说，受精后nanos1的翻译需要死端1（Dnd1），一种脊椎动物特异性生殖系RNA结合蛋白。我们提供的证据表明，Dnd1蛋白，其在卵母细胞中的表达是低的，但显着增加受精后，直接相互作用，并解除抑制功能的真核生物起始因子3f，在43 S preinitiation复合物的抑制成分。这项工作揭示了一种新的翻译调控机制，这对种系发育至关重要。
In the developing embryo, primordial germ cells (PGCs) represent the exclusive progenitors of the gametes, and their loss results in adult infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must transiently silence their genome, an early developmental process that requires Nanos activity. However, it is unclear how Nanos translation is regulated in developing embryos. We report here that translation of nanos1 after fertilization requires Dead-end 1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, expression of which is low in oocytes, but increases dramatically after fertilization, directly interacts with, and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.