Differential effects of sevoflurane on the growth and apoptosis of human cancer cell lines
Differential effects of sevoflurane on the growth and apoptosis of human cancer cell lines
复制标题
DOI：
10.1007/s00540-019-02701-w
复制
发表时间：
2019-12-03
影响因子：
2.8
通讯作者：
Nishiwaki, Kimitoshi
中科院分区：
文献类型：
--
作者：
Hirai, Takahiro;Konishi, Yuko;Nishiwaki, Kimitoshi
目的关于七氟烷对癌细胞致癌特性的影响，存在相互矛盾的研究结果。本研究是为了深入了解七氟烷暴露对各种癌细胞的差异性影响的基本规则，这些癌细胞来自multiple origin.Methods一系列的癌细胞系暴露于1%（v/v）七氟烷2-8 h，然后评估其增殖，Matrigel侵袭和凋亡细胞死亡，与未经处理的对应物相比。分别使用Coulter计数器和Boyden室技术进行细胞增殖和Matrigel侵袭测定。通过用Annexin V和7-AAD染色细胞，然后用荧光流式细胞术评价细胞凋亡。此外，裂解的caspase-3蛋白的表达，另一个凋亡的标志物，进行了评估，使用immunoblotting.Results增殖显着增强七氟烷暴露后，在六个癌细胞系（NCI-H1299，MDA-MB-231，HCT 116，DLD-1，HT 29，和RKO）。相比之下，七氟烷减弱了最后两种癌细胞系A549和MCF-7以及非癌MCF 10A细胞系的增殖。使用四种癌细胞系的细胞生物学测定表明，七氟烷暴露后，加速而不是减弱癌细胞增殖与增强Matrigel侵袭和抑制apoptosis.Conclusion七氟烷增强或阻碍细胞增殖和Matrigel侵袭取决于癌细胞系检查。在癌细胞系中发生的七氟烷诱导的细胞凋亡的丧失可能与七氟烷暴露后其增殖增强相关。
Purpose There have been contradictory findings regarding the effects of sevoflurane on the oncogenic properties of cancer cells. This study was conducted to gain insights into the fundamental rules governing the differential effects of sevoflurane exposure on various cancer cells derived from multiple origins.Methods A series of cancer cell lines were exposed to 1% (v/v) sevoflurane for 2-8 h and then assessed for their proliferation, Matrigel invasion, and apoptotic cell death, in comparison with their untreated counterparts. Cell proliferation and Matrigel invasion assays were performed using Coulter counter and Boyden chamber techniques, respectively. Apoptosis was evaluated by staining cells with Annexin V and 7-AAD followed by fluorescence flow cytometry. In addition, the expression of cleaved caspase-3 protein, another marker of apoptosis, was assessed using immunoblotting.Results Proliferation was significantly enhanced after sevoflurane exposure in six of eight cancer cell lines (NCI-H1299, MDA-MB-231, HCT116, DLD-1, HT29, and RKO). In contrast, sevoflurane attenuated proliferation in the last two cancer cell lines, A549 and MCF-7, as well as in the non-cancerous MCF10A cell line. Cell biological assays using four cancer cell lines demonstrated that accelerated but not attenuated cancer cell proliferation after sevoflurane exposure is associated with enhanced Matrigel invasion and suppressed apoptosis.Conclusion Sevoflurane augmented or hampered cell proliferation and Matrigel invasion depending on the cancer cell line examined. Loss of sevoflurane-induced apoptosis occurring in cancer cell lines is likely to be correlated with their enhanced proliferation after sevoflurane exposure.