Vigna angularis Water Extracts Protect Against Ultraviolet B-Exposed Skin Aging In Vitro and In Vivo
Vigna angularis Water Extracts Protect Against Ultraviolet B-Exposed Skin Aging In Vitro and In Vivo
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DOI：
10.1089/jmf.2013.3017
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发表时间：
2014-12-01
影响因子：
2.4
通讯作者：
Yi, Tae Hoo
中科院分区：
文献类型：
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作者：
Hwang, Eunson;Park, Sang-Yong;Yi, Tae Hoo
暴露于紫外线（UV）辐射诱导各种病理变化，例如皮肤增厚和皱纹形成。特别是，UVB照射增加基质金属蛋白酶（MMP）-1的产生和胶原蛋白降解，导致过早老化，称为光老化。红豆（Vigna angularis; VA）已被广泛用作食品以及传统药物。然而，它的活性需要进一步的研究，以确认其在保护皮肤的食品和化妆品中的功能应用。本研究对VA热水提取物（VAE）及其活性成分芦丁进行了研究，以探讨其抗光老化作用。VAE被发现具有抗氧化活性。在UVB暴露的正常人皮肤成纤维细胞与VAE和芦丁治疗，MMP-1的生产显着抑制（90%和47%，分别）。在UVB照射的无毛小鼠中测试了局部和口服施用VAE的效果。VAE通过促进弹性蛋白、I型前胶原和TGF-β 1的表达（分别为118%、156%和136%）和减少MMP-1的产生来抑制皱纹形成和皮肤厚度。这些结果表明，VAE可能是有效的，以防止皮肤光老化加速UVB辐射。
Exposure to ultraviolet (UV) radiation induces various pathological changes, such as thickened skin and wrinkle formation. In particular, UVB irradiation increases matrix metalloproteinase (MMP)-1 production and collagen degradation, leading to premature aging, termed photoaging. The azuki bean (Vigna angularis; VA) has been widely used as a food product as well as a traditional medicine. However, its activity needs additional study to confirm its functional application in foods and cosmetics for protecting skin. In this study, hot-water extract from VA (VAE) and its active component, rutin, were investigated to determine their antiphotoaging effects. VAE was found to have antioxidant activity. In UVB-exposed normal human dermal fibroblasts cells with VAE and rutin treatments, MMP-1 production was significantly suppressed (90% and 47%, respectively). The effects of both topical and oral administration of VAE were tested in UVB-irradiated hairless mice. VAE suppressed wrinkle formation and skin thickness by promoting elastin, procollagen type I, and TGF-beta 1 expression (118%, 156%, and 136%, respectively) and by diminishing MMP-1 production. These results suggest that VAE may be effective for preventing skin photoaging accelerated by UVB radiation.