Cooperation effects in binding of large ligands to DNA. II. Contact interactions between adsorbed ligands
Cooperation effects in binding of large ligands to DNA. II. Contact interactions between adsorbed ligands
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DOI：
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发表时间：
1984-07
影响因子：
1.2
通讯作者：
Nechipurenko IuD
中科院分区：
文献类型：
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作者：
Nechipurenko IuD
考虑了生物活性配体与DNA结合时产生的协同效应。推导的方程使人们能够描述两个不同的配体与DNA的结合。我们还考虑了配体可以形成两种类型的DNA络合物的情况。对于具有一定有效结合常数的同一配体的非协同吸附，其在结合饱和水平附近的协同结合可以很好地描述。结果表明，蛋白质和其他配体与DNA结合所产生的协同效应可以分为两类，这取决于结合的配体分子之间相互作用的对称性。特别地，如果这种相互作用有利于在DNA上形成二聚体配体物种，则Keff约为A1/2，其中a是配体-配体相互作用常数。如果协同作用有利于形成大小不受限制的聚集体，则Keff近似为al+Y，其中L是配体在DNA上的结合位置的大小。
Cooperative effects arising upon binding of biologically active ligands to DNA are considered. Equations are derived which enable one to describe the binding of two different ligands to DNA. We also consider the case when ligand can form two type of DNA complexes. The cooperative binding of the ligand in the vicinity of saturation level of binding can be described with a good accuracy by equation derived for the non-cooperative adsorption of the same ligand with some effective binding constant Keff. It is shown that cooperative effects arising upon binding of proteins and other ligands to DNA can be divided into two groups depending on the symmetry of interactions between the bound ligand molecules. In particular, if such interactions favor the formation of dimeric ligand species on the DNA, Keff approximately a1/2, where a is the ligand-ligand interaction constant. If cooperative interactions favor the formation of aggregates of unrestricted size, then Keff approximately aL+Y, where L is the size of the binding site for the ligand on DNA.