Distribution of progesterone receptor, estradiol dehydrogenase, and 20 alpha-dihydroprogesterone dehydrogenase activities in human endometrial glands and stroma: progestin induction of steroid dehydrogenase activities in vitro is restricted to the glandular epithelium.
Distribution of progesterone receptor, estradiol dehydrogenase, and 20 alpha-dihydroprogesterone dehydrogenase activities in human endometrial glands and stroma: progestin induction of steroid dehydrogenase activities in vitro is restricted to the glandular epithelium.
复制标题
孕酮受体、雌二醇脱氢酶和20α-二氢孕酮脱氢酶活性在人子宫内膜腺体和间质中的分布：体外孕激素对类固醇脱氢酶活性的诱导仅限于腺上皮。
作者：
P. Satyaswaroop;D. J. Wartell;R. Mortel
通过胶原酶消化人子宫内膜，将子宫内膜腺体与基质细胞分离，并测定这些制剂中孕酮（P）受体的分布以及 P 调节酶 17 β-雌二醇脱氢酶（E2DH）和 20 α-二氢孕酮脱氢酶（20 α DH）的活性。通过对完整增殖子宫内膜以及从该组织分离的腺体和基质细胞中的特异性[3H]P结合进行斯卡查德图分析来估计胞质P受体的浓度。与基质细胞相比，上皮细胞的高亲和力、P 特异性结合位点丰富了 10 倍以上。 [3H]P 结合的结合常数 (Kd) 在未解离的子宫内膜、腺上皮和间质中基本相似，范围为 1--5 nM。在月经周期的增殖和分泌阶段，腺上皮中的 E2DH 和 20 α DH 活性也比整个组织或间质高 3 倍。此外，体外培养的增殖性子宫内膜外植体中孕激素对这些酶活性的诱导仅限于腺上皮。因此，P 对 E2DH 和 20 α DH 的影响在含有 P 受体的相同细胞中表达。
Endometrial glands were separated from stromal cells by collagenase digestion of human endometrium, and the distribution of progesterone (P) receptor and the activities of P-regulated enzymes, 17 beta-estradiol dehydrogenase (E2DH) and 20 alpha-dihydroprogesterone dehydrogenase (20 alpha DH), were determined in these preparations. Concentrations of cytosolic P receptor were estimated by Scatchard plot analysis of specific [3H]P binding in intact proliferative endometrium and in glands and stromal cells isolated from this tissue. Epithelial cells were more than 10-fold enriched in high affinity, P-specific binding sites compared to stromal cells. The binding constants (Kd) for [3H]P binding were essentially similar in undissociated endometrium, glandular epithelium, and stroma, ranging from 1--5 nM. The activities of E2DH and 20 alpha DH were also 3-fold higher in the glandular epithelium than in whole tissue or stroma during both proliferative and secretory stages of the menstrual cycle. In addition, the induction of these enzyme activities by progestin in vitro in cultured explants of proliferative endometrium was restricted to the glandular epithelium. Thus, the effects of P on E2DH and 20 alpha DH are expressed in the same cells that contain receptors for P.