miR‑34a suppresses proliferation and induces apoptosis of human lens epithelial cells by targeting E2F3.
miR‑34a suppresses proliferation and induces apoptosis of human lens epithelial cells by targeting E2F3.
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DOI：
10.3892/mmr.2016.5901
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发表时间：
2016-12
影响因子：
3.4
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Xiang W;Lin H;Wang Q;Chen W;Liu Z;Chen H;Zhang H;Chen W
microRNA（miRNA）在多种疾病中异常表达，与细胞增殖和凋亡密切相关。然而，miRNAs控制白内障发生的机制仍不清楚。在本研究中，通过茎环逆转录-定量聚合酶链反应证实miR-34 a在白内障透镜中高表达。为了研究miR-34 a在人透镜上皮细胞中的作用，将miR-34 a模拟物转染到SRA 01/04细胞中，这抑制了增殖并诱导了凋亡。随后，E2 F3被证实为miR-34 a的直接靶点。小干扰RNA siE 2F 3下调E2 F3表达可导致SRA 01/04细胞增殖抑制和凋亡。此外，证明miR-34 a和siE 2F 3在蛋白水平下调E2 F3表达。综上所述，本研究证实miR-34 a通过下调E2 F3抑制SRA 01/04细胞增殖并诱导其凋亡。这些观察结果为白内障的治疗提供了具有潜在治疗应用的新见解。
microRNA (miRNA) is abnormally expressed in numerous diseases, and it was intimately associated with cell proliferation and apoptosis. However, the mechanism by which miRNAs control cataractogenesis remains unclear. In the current study, it was demonstrated that miR-34a was highly expressed in the cataractous lens by stem-loop reverse transcription-quantitative polymerase chain reaction. Trying to investigate the role of miR-34a in human lens epithelial cells, miR-34a mimics were transfected into SRA01/04 cells, and this suppressed proliferation and induced apoptosis. Subsequently, E2F3 was confirmed as a direct target of miR-34a. Downregulation of E2F3 by small interfering (si) RNA siE2F3 resulted in proliferation inhibition and apoptosis of SRA01/04 cells. Furthermore, it was demonstrated that miR-34a and siE2F3 downregulated E2F3 expression at a protein level. In summary, the current study demonstrated that miR-34a suppressed the proliferation and induced apoptosis of SRA01/04 cells by downregulating E2F3. These observations provide novel insights with potential therapeutic applications for the treatment of cataracts.