LOCALIZATION OF NEUROKININ-B IN THE CENTRAL-NERVOUS-SYSTEM OF THE RAT
LOCALIZATION OF NEUROKININ-B IN THE CENTRAL-NERVOUS-SYSTEM OF THE RAT
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DOI：
10.1016/0196-9781(92)90192-6
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发表时间：
1992-07-01
期刊：
影响因子：
3
通讯作者：
CONLON, JM
中科院分区：
文献类型：
--
作者：
MERCHENTHALER, I;MADERDRUT, JL;CONLON, JM
神经激肽 B (NKB) 的分布通过免疫细胞化学测定，抗血清针对其氨基末端。在主嗅球和副嗅球、皮质区、嗅结节、终纹床核、布罗卡斜带、伏隔核、隔膜、新纹状体、几个下丘脑核、上丘、中央灰质、黑质、髓质网状结构和外楔状核中检测到免疫反应性核周。免疫细胞化学显示的含有NKB的核周细胞的分布与先前通过原位杂交显示的含有原速激肽B的细胞的分布相似。在大脑的所有主要分区中都检测到了免疫反应性神经纤维和末梢。通过放射免疫测定法测得的NK.B水平在下丘脑中最高。 NKB在大鼠脑内的分布与P物质的分布相似；然而，在一些地区，两种分布明显不同。
The distribution of neurokinin B (NKB) was determined by immunocytochemistry with antisera directed toward its amino terminus. Immunoreactive perikarya were detected in the main and accessory olfactory bulbs, cortical regions, the olfactory tubercle, the bed nucleus of the stria terminalis, the diagonal band of Broca, the nucleus accumbens, the septum, the neostriatum, several hypothalamic nuclei, the superior colliculus, the central gray, the substantia nigra the medullary reticular formation, and the external cuneate nucleus. The distribution of NKB-containing perikarya revealed by immunocytochemistry was similar to the distribution of protachykinin B-containing cells previously by in situ hybridization. Immunoreactive nerve fibers and terminals were detected in all major subdivisions of the brain. The levels of NK.B measured by radioimmunoassay were highest in the hypothalamus. The distribution of NKB in the rat brain was similar to the distribution of substance P; however, there were several regions where the two distributions were clearly different.