Immunocompetent cells in the pulp of human deciduous teeth
Immunocompetent cells in the pulp of human deciduous teeth
复制标题
DOI：
10.1016/s0003-9969(03)00173-0
复制
发表时间：
2004-01-01
影响因子：
3
通讯作者：
Yamashita, Y
中科院分区：
文献类型：
--
作者：
Angelova, A;Takagi, Y;Yamashita, Y
本研究通过免疫组织化学方法观察了人乳牙牙髓中各种免疫活性细胞在生理性牙根吸收过程中的分布和密度变化。43个提取的乳牙在不同阶段的吸收进行免疫过氧化物酶染色与针对HLA-DR，CD 68，因子XIIIa和淋巴细胞亚群的抗体的使用。在完整的乳牙（组0），所有类型的细胞检查，除了CD 20 + B淋巴细胞，检测。在吸收小于牙根长度的1/3的牙齿中（第1组），与第0组相比，所有类型的细胞均显示出统计学上显著的增加（P < 0.05; Mann-Whitney's U检验）。HLA-DR+，CD 68+，和因子XIIIa+细胞与树突状分布保持其分布在周围的纸浆，和椭圆形和圆形，新招募的巨噬细胞积累在中央部分的纸浆和附近的吸收网站。在吸收为1/2至2/3的牙齿中（组2），所有细胞类型进一步增加。在牙髓中央部分经常看到HLA-DR+、CD 68+和因子XIIIa+细胞的聚集，T和B淋巴细胞偶尔形成一些簇。与第1组的比较显示，除CD 20+细胞外，这些细胞的密度显示出显著增加（P < 0.05; Mann-Whitney's U-检验）。这些结果提供了证据表明，免疫活性细胞的乳牙牙髓的生理性根吸收的进展，提示乳牙的免疫能力被改变的过程。(C)2003爱思唯尔有限公司。保留所有权利。
This immunohistological study sought to determine how the distribution and density of various immunocompetent cells change in the pulp of human deciduous teeth during the process of physiological root resorption. Forty-three extracted deciduous teeth at various stages of resorption were subjected to immunoperoxiclase staining with the use of antibodies directed to HLA-DR, CD68, factor XIIIa and lymphocyte subsets. In intact deciduous teeth (group 0), all types of cells examined, except for CD20+ B lymphocytes, were detected. In teeth in which resorption was less than 1/3 of the root length (group 1), all types of cells showed a statistically significant increase compared with group 0 (P < 0.05; Mann-Whitney's U-test). HLA-DR+, CD68+, and factor XIIIa+ cells with a dendritic profile kept their distribution in the periphery of the pulp, and oval and round, newly recruited macrophages accumulated in the central portion of the pulp and near the resorption sites. In teeth where resorption was 1/2 to 2/3 (group 2), all the cell types increased further. Aggregations of HLA-DR+, CD68+, and factor XIIIa+ cells were frequently seen in the central portion of the pulp, and T and B lymphocytes occasionally formed some clusters. Comparisons with group 1 revealed that the density of these cells, except for CD20+ cells, showed significant increases (P < 0.05; Mann-Whitney's U-test). These results provided evidence showing that immunocompetent cells of deciduous tooth pulp increase with the progress of physiological root resorption, suggesting that immunocompetency of deciduous teeth is altered by this process. (C) 2003 Elsevier Ltd. All rights reserved.