Preservation and Storage Stability of Extracellular Vesicles for Therapeutic Applications.
Preservation and Storage Stability of Extracellular Vesicles for Therapeutic Applications.
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DOI：
10.1208/s12248-017-0160-y
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发表时间：
2017-11-27
期刊：
影响因子：
--
通讯作者：
Jay SM
中科院分区：
文献类型：
--
作者：
Jeyaram A;Jay SM
近年来，细胞外囊泡(包括外切体、微囊泡等)作为细胞衍生的生物治疗和药物输送载体在各种应用中引起了人们的兴趣。这种兴趣源于电动汽车的良好特性，包括它们作为细胞间通过转移生物货物进行沟通的媒介的地位，以及据报道它们跨越阻碍许多递送系统的生物屏障的能力。然而，以电动汽车为基础的疗法的翻译和广泛应用面临许多挑战。一项尚未得到广泛研究的挑战涉及电动汽车的保存和储存，必须解决这一问题，以便能够在资源密集型环境之外使用治疗性电动汽车。到目前为止的研究表明，最有希望的储存模式是−80°C；然而，对储存中介效应的了解仍然有限。此外，储存的影响似乎因样品来源而异。缺乏关于EV存储的知识和标准化可能最终会阻碍广泛的临床翻译。这篇小型综述报告了电动汽车保存和储存稳定性领域的最新知识，并强调了该领域的未来方向，这可能对电动汽车疗法的最终发展至关重要。
Recently, extracellular vesicles (EVs)—including exosomes, microvesicles, and others—have attracted interest as cell-derived biotherapeutics and drug delivery vehicles for a variety of applications. This interest stems from favorable properties of EVs, including their status as mediators of cell-cell communication via transfer of biological cargo and their reported ability to cross biological barriers that impede many delivery systems. However, there are many challenges to translation and widespread application of EV-based therapeutics. One such challenge that has yet to be extensively studied involves EV preservation and storage, which must be addressed to enable use of therapeutic EVs beyond resource-intensive settings. Studies to date suggest that the most promising mode of storage is −80°C; however, understanding of storage-mediated effects is still limited. Additionally, the effects of storage appear to vary with sample source. The lack of knowledge about and standardization of EV storage may ultimately hinder widespread clinical translation. This mini-review reports current knowledge in the field of EV preservation and storage stability and highlights future directions in the area that could be critical to eventual development of EV therapies.
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DOI：
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