Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin
Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin
复制标题
针对盘基网柄菌肌动蛋白制备的单克隆抗体：表征及其与肌动蛋白的相互作用
作者：
P. A. Simpson;J. Spudich;P. Parham
三种小鼠单克隆抗体，第一，第二，和第四，对肌动蛋白的细胞黏菌Dictyosteelium discoideum，已被提出和特点。所有三种抗体都是IgG 1，并具有以下特性：它们与单体网骨藻肌动蛋白形成稳定的复合物，防止肌动蛋白聚合成细丝。除了预先形成的肌动蛋白丝外，它们还引起肌动蛋白丝尺寸和肌动蛋白溶液粘度的减小。它们与来自低等真核生物绒泡菌和阿米巴的肌动蛋白发生强烈交叉反应，但不与来自兔和人肌肉的α-肌动蛋白或来自人红细胞和人B淋巴细胞系的β-和γ-肌动蛋白发生交叉反应。Act II和Act IV识别相似的抗原决定簇，其在地形上不同于Act I所识别的抗原决定簇。在蛋白质免疫印迹中，只有Act I与Dictyosteoblastrin肌动蛋白强烈结合。用这种技术分析肌动蛋白片段表明，第13至约50位氨基酸是Act I与肌动蛋白结合所必需的。从较低的真核生物和高等脊椎动物的肌动蛋白的氨基酸序列的比较牵连苏氨酸41作为一个关键的残基在Act I抗原位点。Act II和Act IV的性质表明它们识别涉及NH 2-末端6个残基的抗原位点。
Three mouse monoclonal antibodies, Act I, Act II, and Act IV, against actin from the cellular slime mold Dictyostelium discoideum, have been made and characterized. All three antibodies are IgG1 and share the following properties: They form stable complexes with monomeric Dictyostelium actin, which prevents polymerization of the actin into filaments. On addition to preformed actin filaments, they cause a reduction in filament size and in the viscosity of the actin solution. They cross-react strongly with actins from the lower eucaryotes Physarum and Acanthamoeba, but not with alpha-actins from rabbit and human muscle or beta- and gamma-actins from human erythrocytes and a human B lymphoid cell line. Act II and Act IV recognize a similar antigenic determinant that is topographically distinct from that identified by Act I. In protein immunoblotting, only Act I bound strongly to Dictyostelium actin. Analysis of actin fragments with this technique showed that amino acids 13 to about 50 are required for Act I binding to actin. A comparison of the amino acid sequences of actins from lower eucaryotes and higher vertebrates implicates threonine 41 as a critical residue in the Act I antigenic site. The properties of Act II and Act IV suggest that they recognize antigenic sites involving the NH2-terminal six residues.
针对 HLA 产品的单克隆抗体及其在免疫亲和纯化中的应用。
影响因子：
--
作者：
Parham,P
通讯作者：
Parham,P
骨骼肌肌动蛋白的蛋白水解和结构。
DOI：
10.1073/pnas.81.12.3680
发表时间：
1984
影响因子：
11.1
作者：
Mornet,D;Ue,K
通讯作者：
Ue,K