Characterization of membrane-associated Clostridium perfringens enterotoxin following pronase treatment
Characterization of membrane-associated Clostridium perfringens enterotoxin following pronase treatment
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DOI：
10.1128/iai.66.12.5897-5905.1998
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发表时间：
1998-12-01
影响因子：
3.1
通讯作者：
McClane, BA
中科院分区：
文献类型：
--
作者：
Wieckowski, EU;Kokai-Kun, JF;McClane, BA
结合后，产气荚膜梭菌肠毒素（CPE）最初定位于质膜中的小（类似于90-kDa）复合物中。该事件之后是第二个膜复合物的形成，称为大（160-kDa）复合物。与先前的假设相反，提出CPE插入肠刷状缘膜（BBM）时，这种毒素是本地化的小复杂的，这项研究表明，BBM不提供CPE本地化的小复杂的保护链霉蛋白酶。然而，我们的实验表明，BBM确实在很大程度上保护CPE免受链霉蛋白酶的侵害，当这种毒素位于大的复合物中时。由于CPE诱导的渗透性改变的发病密切符合大复杂的形成，这些新的结果表明，CPE诱导的渗透性改变可能会导致孔形成由于CPE的部分膜插入时，这种毒素是存在于大复杂。
After binding, Clostridium perfringens enterotoxin (CPE) initially localizes in a small (similar to 90-kDa) complex in plasma membranes. This event is followed by formation of a second membrane complex, referred to as large (160-kDa) complex. Contrary to a previous hypothesis proposing that CPE inserts into intestinal brush border membranes (BBMs) when this toxin is localized in the small complex, this study shows that BBMs do not offer CPE localized in the small complex protection from pronase. However, our experiments indicate that BBMs do substantially protect CPE from pronase when this toxin is localized in large complex. Since the onset of CPE-induced permeability alterations closely coincides with large-complex formation, these new results suggest that CPE-induced alterations in permeability may result from pore formation due to the partial membrane insertion of CPE when this toxin is present in large complex.