Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking.
Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking.
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DOI：
10.2217/nnm.14.134
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发表时间：
2015-02
期刊：
影响因子：
5.5
通讯作者：
Gabriel D. Pemberton;Peter G. Childs;S. Reid;H. Nikukar;P. Tsimbouri;N. Gadegaard;A. Curtis;M. Dalby
中科院分区：
文献类型：
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作者：
Gabriel D. Pemberton;Peter G. Childs;S. Reid;H. Nikukar;P. Tsimbouri;N. Gadegaard;A. Curtis;M. Dalby
目的间充质干细胞(MSCs)具有较大的再生潜能，可替代中胚层沿线多个组织的受损细胞。这些细胞的潜能有望改变我们老龄化人口目前遭受的许多退行性疾病的长期预后。我们努力展示了我们在垂直方向上使用高频(1000-5000赫兹)压电驱动的纳米位移(16-30 nm位移)来诱导MSCs成骨的能力。材料与方法通过实时定量聚合酶链式反应检测成骨基因ONN、RUNX2和Osterix的表达，在蛋白质水平检测骨钙素(OCN)和骨桥蛋白(OPN)的表达，通过组织学染色检测磷酸钙的沉积，以确定成骨细胞的形成。结果有趣的是，我们观察到了纳米拓扑学和压电刺激的机械转导之间的关系，并可能看到两种不同的成骨机制在起作用的证据。这些数据为干细胞分化的纳米机制转导提供了信心，而不依赖于可溶性因素和复杂的化学成分。结论在未来，这项技术有望在医疗保健行业中得到有益的治疗应用，这些疾病的总体表型可能以脆弱或受损的骨骼为特征(如骨质疏松和骨折)，并且可以受益于体内成骨细胞的数量增加。
AIM Mesenchymal stem cells (MSCs) have large regenerative potential to replace damaged cells from several tissues along the mesodermal lineage. The potency of these cells promises to change the longer term prognosis for many degenerative conditions currently suffered by our aging population. We have endeavored to demonstrate our ability to induce osteoblatogenesis in MSCs using high-frequency (1000-5000 Hz) piezo-driven nanodisplacements (16-30 nm displacements) in a vertical direction. MATERIALS & METHODS Osteoblastogenesis has been determined by the upregulation of osteoblasic genes such as osteonectin (ONN), RUNX2 and Osterix, assessed via quantitative real-time PCR; the increase of osteocalcin (OCN) and osteopontin (OPN) at the protein level and the deposition of calcium phosphate determined by histological staining. RESULTS Intriguingly, we have observed a relationship between nanotopography and piezo-stimulated mechanotransduction and possibly see evidence of two differing osteogenic mechanisms at work. These data provide confidence in nanomechanotransduction for stem cell differentiation without dependence on soluble factors and complex chemistries. CONCLUSION In the future it is envisaged that this technology may have beneficial therapeutic applications in the healthcare industry, for conditions whose overall phenotype maybe characterized by weak or damaged bones (e.g., osteoporosis and bone fractures), and which can benefit from having an increased number of osteoblastic cells in vivo.