SEX-DIFFERENCES IN THE ULTRADIAN PATTERN OF PLASMA GROWTH-HORMONE CONCENTRATIONS IN MICE
SEX-DIFFERENCES IN THE ULTRADIAN PATTERN OF PLASMA GROWTH-HORMONE CONCENTRATIONS IN MICE
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DOI：
10.1677/joe.0.1310395
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发表时间：
1991-12-01
影响因子：
4
通讯作者：
SHAPIRO, BH
中科院分区：
文献类型：
--
作者：
MACLEOD, JN;PAMPORI, NA;SHAPIRO, BH
测定成年雄性和雌性小鼠血浆 GH 浓度的超电模式。通过手术放置的慢性留置右心房导管，在 8 小时内每 15 分钟收集一次连续血样，并通过同源放射免疫测定法测定 GH 含量。在两性中，GH 浓度从基线值（通常在 2-mu-g/l 范围内，接近测定灵敏度的极限）周期性波动，到峰值（有时达到 100-mu-g/l）。然而，雄性小鼠表现出大约每 2.5 小时 GH 峰值的规律性周期性，并插入稳定的基线浓度，其持续时间明显长于雌性小鼠。女性中缺乏延长的基线浓度将周期长度缩短至平均 1.4 小时，并显着增加了总体平均 GH 浓度。男性和女性的 GH 峰值持续时间、高度和面积以及峰值之间的平均浓度相当。在小鼠和大鼠中，血清 GH 浓度超电模式的性别二态性已被证明可以调节几种性别特异性表型的表达。比较小鼠和大鼠的内源性 GH 模式表明，在相同的时间间隔内，这两个物种的雄性的峰值频率低于雌性。 GH 峰的这种分离似乎是表达男性 GH 依赖性性状的基本要素。
Ultradian patterns of plasma GH concentration were determined in adult male and female mice. Serial blood samples were collected every 15 min over 8 h through surgically placed chronic indwelling right atrial catheters and assayed for GH content by an homologous radioimmunoassay. In both sexes, GH concentrations fluctuated episodically from baseline values that were often in the range of 2-mu-g/l which approached the limit of assay sensitivity, to peak values sometimes reaching 100-mu-g/l. Male mice, however, demonstrated a regular periodicity of GH peaks approximately every 2.5 h with interposed stable baseline concentrations that were significantly longer in duration than in females. The absence of extended baseline concentrations in females reduced cycle length to an average of 1.4 h and significantly increased the overall mean GH concentration. The duration, height and area of GH peaks and the average concentration between peaks were comparable in males and females. Sexual dimorphism in the ultradian patterns of serum GH concentrations have been shown in both mice and rats to regulate the expression of several sex-specific phenotypes. Comparing endogenous GH patterns in mice and rats demonstrates that males of both species have less frequent peaks than females over the same interval of time. This separation of GH peaks appears to be an essential element for the expression of masculine GH-dependent traits.