Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex
Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex
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DOI：
10.1097/01.wcb.0000122745.72175.d5
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发表时间：
2004-07-01
影响因子：
6.3
通讯作者：
Moskowitz, MA
中科院分区：
文献类型：
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作者：
Ayata, C;Dunn, AK;Moskowitz, MA
激光散斑血流仪（LSF）有助于以高时空分辨率无创地评估二维脑血流（CBF）。作者表明，LSF能够通过完整的颅骨对小鼠脑血流变化的时空动态进行成像。当用LSF测量时，在触须刺激期间脑血流峰值的增加与同时进行的激光多普勒血流仪（LDF）测量密切相关，并且在供应桶状皮层的大脑中动脉分支内比在桶状皮层毛细血管床本身内增加得更多。当使用LSF研究对吸入5%二氧化碳的反应时，血流增加与使用LDF所报道的反应相似。对于自动调节的上限和下限，平均动脉压值分别为110和40 mmHg。他们还表明，通过[C - 14]碘安非他明技术测定的绝对静息脑血流与使用LSF获得的1/τ(c)值之间存在线性关系，并使用1/τ(c)值来比较不同动物之间的静息脑血流。最后，作者研究了大脑中动脉远端结扎后的脑血流变化，并建立了一个模型来研究中度至重度缺血皮层的空间分布和血流动力学。总之，由于具有高空间分辨率，LSF在脑血流监测方面比LDF具有明显优势。
Laser speckle flowmetry (LSF) is useful to assess noninvasively two-dimensional cerebral blood flow (CBF) with high temporal and spatial resolution. The authors show that LSF can image the spatiotemporal dynamics of CBF changes in mice through an intact skull. When measured by LSF, peak CBF increases during whisker stimulation closely correlated with simultaneous laser-Doppler flowmetry (LDF) measurements, and were greater within the branches of the middle cerebral artery supplying barrel cortex than within barrel cortex capillary bed itself. When LSF was used to study the response to inhaled CO2 (5%), the flow increase was similar to the response reported using LDF. For the upper and lower limits of autoregulation, mean arterial pressure values were 110 and 40 mm Hg, respectively. They also show a linear relationship between absolute resting CBF, as determined by [C-14]iodoamphetamine technique, and 1/tau(c) values obtained using LSF, and used 1/tau(c) values to compare resting CBF between different animals. Finally, the authors studied CBF changes after distal middle cerebral artery ligation, and developed a model to investigate the spatial distribution and hemodynamics of moderate to severely ischemic cortex. In Summary, LSF has distinct advantages over LDF for CBF monitoring because of high spatial resolution.