Effects of Fluoride on Oxidative Stress Markers of Lipid, Gene, and Protein in Rats.
Effects of Fluoride on Oxidative Stress Markers of Lipid, Gene, and Protein in Rats.
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氟化物对大鼠脂质、基因和蛋白质氧化应激标志物的影响。
DOI：
10.1007/s12011-020-02336-z
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发表时间：
2021
影响因子：
3.9
通讯作者：
Pei Junrui
中科院分区：
文献类型：
--
作者：
Zhong Nan;Yao Yingjie;Ma Yongzheng;Meng Xinyue;Sowanou Alphonse;Pei Junrui
地方性氟中毒是因摄入过量氟而引起的一种全身性慢性疾病。人们普遍认为氧化应激与氟中毒密切相关；然而，氟中毒中氧化应激的分子机制仍不清楚。本研究探讨氟化物（F）对大鼠脂质、基因和蛋白质氧化应激标志物的影响，以揭示氟中毒氧化应激的分子机制。结果表明，氟化物浓度和暴露时间对MDA和8-OHdG都有显着影响。氟化物浓度显着影响 AGE 水平，但暴露时间却没有影响。 AOPP 在各组之间没有统计学差异。氟治疗3个月的大鼠，随着氟的增加，AGEs降低。相关分析显示，氟斑牙程度与1个月、3个月时的8-OHdG呈显着负相关，与3个月时的AGEs呈显着负相关。 100mg/L氟处理大鼠中，MDA与8-OHdG呈显着正相关，与AGEs呈显着负相关。对照组和100 mg/L氟组8-OHdG与AGEs呈显着负相关。总的来说，氟化物对脂质、基因和蛋白质的氧化应激标志物有不同的影响。过量的氟化物会增加MDA含量，并降低8-OHdG和AGEs。这些发现表明氧化应激参与氟中毒的分子发病机制是复杂的，未来需要进一步研究。
Endemic fluorosis is a systemic chronic disease caused by excessive intake of fluoride. It is widely accepted that oxidative stress is closely related to fluorosis; however, molecular mechanism of oxidative stress in fluorosis remains unclear. This study investigated the effects of fluoride (F) on oxidative stress markers of lipid, gene, and protein in rats for revealing molecular mechanism of oxidative stress in fluorosis. The results showed concentration and exposure time of fluoride both had a significant effect on MDA and 8-OHdG. Fluoride concentration significantly impacted AGEs level, but exposure time did not. AOPP was not statistically different among the groups. AGEs decreased with the increase of fluoride in the rats with 3 months of fluoride treatment. The correlation analysis showed the degree of dental fluorosis was significantly negatively correlated with 8-OHdG at 1 month and 3 months, and negatively correlated with AGEs at 3 months. In the rats with 100 mg/L of fluoride treatment, MDA was significant positively correlated with 8-OHdG, and negatively correlated with AGEs. 8-OHdG was significantly negatively correlated with AGEs in the control group and 100 mg/L fluoride group. Taken together, fluoride had different effects on oxidative stress markers of lipid, gene, and protein. Excessive fluoride could increase MDA content, and decrease 8-OHdG and AGEs. These findings suggest that oxidative stress involved in molecular pathogenesis of fluorosis is complicated, and needs to furtherly study in the future.