A Novel 3D Scaffold for Cell Growth to Assess Electroporation Efficacy
A Novel 3D Scaffold for Cell Growth to Assess Electroporation Efficacy
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DOI：
10.3390/cells8111470
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发表时间：
2019-11-01
期刊：
影响因子：
6
通讯作者：
Signori, Emanuela
中科院分区：
文献类型：
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作者：
Dettin, Monica;Sieni, Elisabetta;Signori, Emanuela
肿瘤电穿孔(EP)是指通过短的电脉冲使细胞膜通透，从而增强化疗药物的作用。由于缺乏细胞-细胞相互作用和细胞外基质(ECM)，标准平板贴壁2D细胞培养只能部分模拟体内环境。在这项研究中，我们通过研究两种不同的乳腺癌细胞株(HCC1569和MDA-MB231)的生长模式，评估了一种基于透明质酸和离子互补自组装肽(SAPS)的新型3D细胞培养支架。这种3D支架调节细胞形状，并诱导细胞周围的细胞外基质沉积。在MDA-MB 231细胞系中，它允许被称为球体的结构的三维生长，而在HCC1569中，它实现了与体内观察到的相似的细胞组织。有趣的是，我们能够通过标准的碘化丙啶分析和荧光显微镜观察到种植在新支架上的细胞的电穿孔效应。由于细胞-细胞和细胞-ECM相互作用的存在，新的3D支架可能比2D癌细胞培养更可靠地支持EP研究，并可用于测试新的EP释放药物和新的EP方案。
Tumor electroporation (EP) refers to the permeabilization of the cell membrane by means of short electric pulses thus allowing the potentiation of chemotherapeutic drugs. Standard plate adhesion 2D cell cultures can simulate the in vivo environment only partially due to lack of cell-cell interaction and extracellular matrix (ECM). In this study, we assessed a novel 3D scaffold for cell cultures based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs), by studying the growth patterns of two different breast carcinoma cell lines (HCC1569 and MDA-MB231). This 3D scaffold modulates cell shape and induces extracellular matrix deposit around cells. In the MDA-MB 231 cell line, it allows three-dimensional growth of structures known as spheroids, while in HCC1569 it achieves a cell organization similar to that observed in vivo. Interestingly, we were able to visualize the electroporation effect on the cells seeded in the new scaffold by means of standard propidium iodide assay and fluorescence microscopy. Thanks to the presence of cell-cell and cell-ECM interactions, the new 3D scaffold may represent a more reliable support for EP studies than 2D cancer cell cultures and may be used to test new EP-delivered drugs and novel EP protocols.