CELL PROLIFERATION AND MIGRATION AS REVEALED BY RADIOAUTOGRAPHY AFTER INJECTION OF THYMIDINE-H-3 INTO MALE RATS AND MICE
CELL PROLIFERATION AND MIGRATION AS REVEALED BY RADIOAUTOGRAPHY AFTER INJECTION OF THYMIDINE-H-3 INTO MALE RATS AND MICE
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DOI：
10.1002/aja.1001060305
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发表时间：
1960-01-01
影响因子：
--
通讯作者：
LEBLOND, CP
中科院分区：
文献类型：
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作者：
MESSIER, B;LEBLOND, CP
将胸腺嘧啶核苷-H3 注射到幼年大鼠和小鼠中，随后 20 分钟至 3 个月不等的时间建立放射自显影分布。注射后立即测定摄取位点，并在稍后的时间间隔跟踪标记细胞的迁移（如果有）。根据胸苷-H3的摄取，200克大鼠的细胞群可以分为3类： (a)静态：其中没有出现标记的细胞核。该组包括各种神经元和神经视网膜。 (b) 膨胀：其中出现少量标记的细胞核并无限期地持续存在，表明新细胞永久地添加到细胞群中，从而使细胞体积增大（其速度与体重本身膨胀的速度相当）。这一类包括肌肉组织（平滑肌、心肌和横纹）、大多数结缔组织、肝脏和肾脏等器官、胰腺和舌下腺等腺体、甲状腺和肾上腺等内分泌腺、晶状体上皮细胞等。（c）更新：其中大量标记细胞核的出现表明细胞生成活跃，而随后标记细胞核的快速减少和消失表明细胞损失。细胞的同时产生和损失将使种群不断更替。该类别包括 2 种类型的种群：其中一些种群的增殖发生在限制区域，例如：复层鳞状上皮的基底区、胃表面细胞的胃腺峡部、小肠和大肠的利伯基林隐窝等；以及其他扩散扩散的情况，例如精囊上皮、造血细胞等...
Thymidine-H3 was injected to young rats and mice and the radioautographic distribution established at times varying from 20 minutes to 3 months thereafter. The sites of uptake were determined immediately after injection and the migration of labelled cells, if any, was followed at later intervals. The cell populations of the 200-g rat may be classified into 3 categories on the basis of thymidine-H3 uptake: (a) static: in which no labelled nuclei appear. This group includes various neurones and the nervous retina. (b) expanding: in which a small number of labelled nuclei appear and persist indefinitely, indicating that new cells are permanently added to the cell population, which thus increases in size (at a rate comparable to that at which the body weight itself is expanding). This category includes muscle tissues (smooth, cardiac and striated), most connective tissues, organs such as liver and kidney, glands such as pancreas and sublingual, endocrines such as thyroid and adrenal, the epithelial cells of the lens, etc.(c) renewing: in which the appearance of large numbers of labelled nuclei indicates active cell production, while the subsequent rapid decrease and disappearance of the labelled nuclei indicate cell loss. Simultaneous production and loss of cells would allow a continuous replacement of the population. This category includes 2 types of populations: some in which proliferation occurs in restricted areas, e.g. the basal region in stratified squamous epithelia, the isthmus of gastric glands for surface cells of stomach, the crypts of Lieberkilhn for small and large intestine, etc.; and others in which proliferation is diffuse, e.g. seminal vesicle epithelium, blood forming cells, etc...