Myosin heavy-chain isoforms in human smooth muscle.
Myosin heavy-chain isoforms in human smooth muscle.
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人平滑肌中的肌球蛋白重链亚型。
DOI：
--
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
S. Schiaffino
中科院分区：
文献类型：
--
作者：
S. Sartore;N. de Marzo;A. Borrione;A. Zanellato;L. Saggin;L. Fabbri;S. Schiaffino
The myosin heavy-chain composition of human smooth muscle has been investigated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, enzyme immunoassay, and enzyme-immunoblotting procedures. A polyclonal and a monoclonal antibody specific for smooth muscle myosin heavy chains were used in this study. The two antibodies were unreactive with sarcomeric myosin heavy chains and with platelet myosin heavy chain on enzyme immunoassay and immunoblots, and stained smooth muscle cells but not non-muscle cells in cryosections and cultures processed for indirect immunofluorescence. Two myosin heavy-chain isoforms, designated MHC-1 and MHC-2 (205 kDa and 200 kDa, respectively) were reactive with both antibodies on immunoblots of pyrophosphate extracts from different smooth muscles (arteries, veins, intestinal wall, myometrium) electrophoresed in 4% polyacrylamide gels. In the pulmonary artery, a third myosin heavy-chain isoform (MHC-3, 190 kDa) electrophoretically and antigenically distinguishable from human platelet myosin heavy chain, was specifically recognized by the monoclonal antibody. Analysis of muscle samples, directly solubilized in a sodium dodecyl sulfate solution, and degradation experiments performed on pyrophosphate extracts ruled out the possibility that MHC-3 is a proteolytic artefact. Polypeptides of identical electrophoretic mobility were also present in the other smooth muscle preparations, but were unreactive with this antibody. The presence of three myosin heavy-chain isoforms in the pulmonary artery may be related to the unique physiological properties displayed by the smooth muscle of this artery.
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DOI：
10.1016/s0021-9258(17)42610-x
发表时间：
1984-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
V. Fatigati;Richard;Murphy
通讯作者：
V. Fatigati;Richard;Murphy
来自小腿主动脉平滑肌的肌球蛋白聚合。
影响因子：
2.9
作者：
Megerman,J;Lowey,S
通讯作者：
Lowey,S
平滑肌肌球蛋白的构象状态。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Trybus,KM;Lowey,S
通讯作者：
Lowey,S
体内血管平滑肌肌球蛋白抗原表达的异质性。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Larson,DM;Fujiwara,K;Alexander,RW;GimbroneJr,MA
通讯作者：
GimbroneJr,MA